ROCK1 Antibody

Rho Kinase Beta, a 160 kDa small-GTP-effector protein, is a serine/threonine kinase involved in the induction of focal adhesions and stress fibers, and can play a major role in vascular smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis. ROCK1 is identified as a substrate for caspases and is cleaved by caspase 3 at a conserved DETD1113/G sequence which removes its carboxy-terminal inhibitory domain, resulting in a constitutively active 130 kDa ROCK1 kinase. Proteolytic activation of ROCK1 by caspase is critical for its role in regulating the actin cytoskeleton and Myosin Light Chain (MLC) phosphorylation. Increased phosphorylation of MLC is important for membrane blebbing, a cellular event that occurs at the onset of apoptosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.

Showing Publications 1 - 3 of 3.

Publications using NB100-624	Applications	Species
Ebhardt HA, Degen S, Tadini V et al. Comprehensive proteome analysis of human skeletal muscle in cachexia and sarcopenia: A pilot study. J Cachexia Sarcopenia Muscle. 2017-03-15 [PMID: 28296247] (WB, Human)	WB	Human
Gilkes DM, Xiang L, Lee SJ et al. Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells. Proc Natl Acad Sci U S A 2014-01-21 [PMID: 24324133] (WB, IHC-P, ICC/IF, IF/IHC, Human)	WB, IHC-P, ICC/IF, IF/IHC	Human
Chang J, Xie M, Shah VR et al. Activation of Rho-associated coiled-coil protein kinase 1 (ROCK-1) by caspase-3 cleavage plays an essential role in cardiac myocyte apoptosis. Proc Natl Acad Sci U S A 2006-09-01 [PMID: 16983089]

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IP	Mouse	04/09/2018	View Summary Application Immunoprecipitation Sample Tested MC3T3-E1 cell Species Mouse Lot A2
Enlarge	5 reviewed by: Verified Customer	WB	Human	08/17/2012	View Summary Application Western Blot Sample Tested MDA-MB-231 Cell Lysate Species Human

Array NB100-624

• ROCK1 Antibodies
• ROCK1 Lysate
• ROCK1 Proteins
• ROCK1 Proteins and Enzyme