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RIG-I Antibody (6K8P2)

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Western Blot: RIG-I Antibody (6K8P2) [NBP3-33530] - Western blot analysis of various lysates, using RIG-I Rabbit mAb at 1:1000 dilution. THP-1 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
Western Blot: RIG-I Antibody (6K8P2) [NBP3-33530] - Western blot analysis of various lysates, using RIG-I Rabbit mAb at 1:1000 dilution. THP-1 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.Secondary antibody: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
6K8P2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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RIG-I Antibody (6K8P2) Summary

Immunogen
A synthesized peptide derived from human RIG-I.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DDX58
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
Theoretical MW
107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RIG-I Antibody (6K8P2)

  • DDX58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEAD box protein 58
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • DKFZp686N19181
  • EC 3.6.4.13
  • FLJ13599
  • probable ATP-dependent RNA helicase DDX58
  • retinoic acid inducible gene I
  • Retinoic acid-inducible gene 1 protein
  • Retinoic acid-inducible gene I protein
  • RIG-1
  • RIGI
  • RIG-I
  • RIG-IDKFZp434J1111
  • RNA helicase RIG-I

Background

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
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Bioinformatics

Gene Symbol DDX58