Flow Cytometry: RHAMM/CD168 Antibody [Alexa Fluor® 700] [NBP1-76538AF700] - Human megakaryocytes. 47,000 CD41+ CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20 min at room ...read more
Antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM. The immunogen is located within amino acids 80 - 130 of RHAMM. Amino Acid Squence: ERGAQDRRIQDLETELEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMMR
Purity
Peptide affinity purified
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Optimal dilution of this antibody should be experimentally determined. RHAMM/CD168 Antibody [Alexa Fluor(R) 700] validated for Flow cytometry from a verified customer review.
Reviewed Applications
Read 1 Review rated 5 using NBP1-76538AF700 in the following applications:
Porcine reactivity reported in scientific literature (PMID: 27856308).
Packaging, Storage & Formulations
Storage
Store at 4C in the dark.
Buffer
50 mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified
Notes
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for RHAMM/CD168 Antibody [Alexa Fluor® 700]
CD168 antigen
CD168
HMMR
hyaluronan mediated motility receptor
hyaluronan-mediated motility receptor (RHAMM)
IHABP
Intracellular hyaluronic acid-binding protein
MGC119494
Receptor for hyaluronan-mediated motility
RHAMM
RHAMMMGC119495
Background
The hyaluronan-mediated motility receptor, also known as RHAMM, was initially identified as a soluble protein that could be released by sub-confluent migrating cells, promoting cell motility and invasion via interactions with hyaluronan (HA) and the cell surface. While RHAMM is normally poorly expressed in most normal tissues and is not required for embryonic development or normal cell homeostasis functions, its expression is increased during wound repair in response to hypoxia and fibrogenic factors. However, its overexpression is transforming in multiple types of cancers and is required for maintaining RAS transformation. RHAMM associates with BRCA1 and BARD1, attenuating the mitotic-spindle-promoting activity of RHAMM, which may contribute to tumor progression by promoting genomic instability.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
47,000 CD41+CD42+ human iPS-derived megakaryocytes [Moreau et al., 2016] were stained for 20min at room temperature with the indicated quantity of antibody. Cells were then washed in 500uL PBS/BSA/EDTA and viable DAPI- cells were analysed using a Galios flow cytometer.
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