![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining in human testis and liver tissues using anti-RFX3 antibody. Corresponding RFX3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RFX3-Antibody-Immunohistochemistry-Paraffin-NBP1-86301-img0011.jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining in human testis and liver tissues using anti-RFX3 antibody. Corresponding RFX3 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: QYMAMRQQPMQQKQRYKPMQKVDGVADGFTGSGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDIKSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESSNLSE |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RFX3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RFX3 |
![Western Blot: RFX3 Antibody [NBP1-86301] - Analysis in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/RFX3-Antibody-Western-Blot-NBP1-86301-img0007.jpg "Western Blot: RFX3 Antibody [NBP1-86301] - Analysis in control (vector only transfected HEK293T lysate) and rFX3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: RFX3 Antibody [NBP1-86301] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RFX3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86301-img0008.jpg "Immunocytochemistry/Immunofluorescence: RFX3 Antibody [NBP1-86301] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/RFX3-Antibody-Immunohistochemistry-Paraffin-NBP1-86301-img0010.jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/RFX3-Antibody-Immunohistochemistry-Paraffin-NBP1-86301-img0009.jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] - Staining of human liver shows low expression as expected.")
![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-86301_rabbit-polyclonal-rfx3-antibody-immunohistochemistry-paraffin-151220239939..jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-86301_rabbit-polyclonal-rfx3-antibody-immunohistochemistry-paraffin-1512202391040..jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-86301_rabbit-polyclonal-rfx3-antibody-immunohistochemistry-paraffin-1512202391146..jpg "Immunohistochemistry-Paraffin: RFX3 Antibody [NBP1-86301] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
![Immunocytochemistry FoxJ1/HFH4 Antibody [Unconjugated]](https://images.novusbio.com/images2/FoxJ1_AF3619_Immunocytochemistry_15937.jpg)
![Immunocytochemistry FoxJ1/HFH4 Antibody [Unconjugated]](https://images.novusbio.com/images2/FoxJ1_AF3619_Immunocytochemistry_10943.jpg)
![Simple Western FoxJ1/HFH4 Antibody [Unconjugated]](https://images.novusbio.com/images2/FoxJ1_AF3619_Simple_Western_22131.jpg)
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![Immunohistochemistry Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2156_human-mouse-nurr1-ngfi-b-beta-nr4a2-affinity-purified-pab-immunohistochemistry-10122021111228.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
![Bioactivity FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF8_423F8_1438.jpg)
![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
![Immunohistochemistry FGF acidic/FGF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/FGF_acidic_AF232_Immunohistochemistry_17183.jpg)
![Simple Western FGF acidic/FGF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/FGF_acidic_AF232_Simple_Western_17471.jpg)
![Western Blot FGF acidic/FGF1 Antibody [Unconjugated]](https://images.novusbio.com/images2/FGF_acidic_AF232_Western_Blot_18640.jpg)
![Flow Cytometry Pax5/BSAP Antibody (1207C) [Unconjugated]](https://images.novusbio.com/images2/Pax5_MAB3487_Flow_Cytometry_19328.jpg)
![Western Blot Pax5/BSAP Antibody (1207C) [Unconjugated]](https://images.novusbio.com/images2/Pax5_MAB3487_Western_Blot_19363.jpg)
![Immunohistochemistry Pax5/BSAP Antibody (1207C) [Unconjugated]](https://images.novusbio.com/images2/Pax5_MAB3487_Immunohistochemistry_19379.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")