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Human RENBP ELISA Kit (Colorimetric)

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ELISA: Human RENBP ELISA Kit (Colorimetric) [KA1838] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Plasma, Serum
Standard Curve Range
0.195 to 50 ug/mL
Sensitivity
0.17 ug/mL

Order Details

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Human RENBP ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard curve. RENBP (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of human RENBP.
Standard Curve Range
0.195 to 50 ug/mL
Sensitivity
0.17 ug/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 7.2%
Intra-Assay
CV% < 4.5%
Spike Recovery
97%
Sample Volume
25 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
RENBP

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated RBP: 1 vial, lyophilized.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine (8 mL).
  3. MIX Diluent Concentrate (10×): A 10-fold concentrated buffered protein base (30 mL).
  4. RBP Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human RBP.
  5. RBP Standard: Human RBP in a buffered protein base (100 µg, lyophilized).
  6. Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
  7. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 mL).
  8. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (80 µL).
  9. Wash Buffer Concentrate (20×): A 20-fold concentrated buffered surfactant (30 mL, 1 bottle).

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human RENBP ELISA Kit (Colorimetric)

  • AGE
  • EC 5.1.3.8
  • GlcNAc 2-epimerase
  • N-acetyl-D-glucosamine 2-epimerase
  • N-acylglucosamine 2-epimerase
  • RBP
  • renin binding protein
  • Renin-binding protein
  • RNBP

Background

RENBP, also known as N-acylglucosamine 2-epimerase, is a 49kDa 427 amino acid protein with a shorter 29kDa 254 isoform produced by alternative splicing. RENBP functions as a catalyzer in the conversion of N-acetylglucosamine to N-acetylmannosamine. Current research is being conducted on RENBP in relation to sialuria, Wilms tumor, allergic rhinitis, myopathy, vascular disease, hypertension, nephropathy, proteinuria and aldosteronism. RENBP has been linked to the amino sugar and nucleotide sugar metabolism pathways where it interacts with HEXA, HEXB, GNE, REN and ZBED1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RENBP