RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set

NF-kB has been shown to regulate the expression of a number of genes whose products are involved in inflammation, viral replication, carcinogenesis, anti-apoptosis, invasion, and metastasis. Specific adhesion molecules, chemokines, inflammatory cytokines, and cell cycle regulatory genes are affected. Thus, agents that can suppress NF-kB activation have the potential to be treatments for inflammatory diseases and cancer.

The Ser529/536 sites of p65 are phosphorylated during NF-kB activation, allowing p65 nuclear translocation. This p65 inhibitory peptide contains Ser529/536 sites that are phosphorylated during NF-kB activation, thereby blocking p65 Ser529/536 phosphorylation. The NF-kB p65(Ser529/536) Inhibitor Peptide can inhibit binding of recombinant p65 protein to the DNA in a dose dependent manner and maximum inhibition occurs at 50 uM (Figure 1). It can also inhibit TNF-induced NF-kB activation in cells (See Figure 2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NBP2-29321	Applications	Species
Almarghlani A, Settem RP, Croft AJ et al. Interleukin-34 permits Porphyromonas gingivalis survival and NF-?B p65 inhibition in macrophages Molecular Oral Microbiology 2022-06-01 [PMID: 35576119]
Sherman LS, Patel SA, Castillo MD Et al. Nf kappa B Targeting in Bone Marrow Mesenchymal Stem Cell-Mediated Support of Age-Linked Hematological Malignancies Stem cell reviews and reports 2021-08-19 [PMID: 34410592]
Yang D, Li S, Duan X TLR4 induced Wnt3a-Dvl3 restrains the intensity of inflammation and protects against endotoxin-driven organ failure through GSK3beta/beta-catenin signaling Mol. Immunol. 2019-12-26 [PMID: 31884387] (Human, Mouse)		Human, Mouse
Mukohda M, Lu KT, Guo DF et al. Hypertension-Causing Mutation in Peroxisome Proliferator-Activated Receptor y Impairs Nuclear Export of Nuclear Factor-kB p65 in Vascular Smooth Muscle. Hypertension 2017-05-15 [PMID: 28507170] (Mouse)		Mouse
Mukohda M, Stump M, Ketsawatsomkron P et al. Endothelial PPARy Provides Vascular Protection from IL-1B-Induced Oxidative Stress. Am. J. Physiol. Heart Circ. Physiol. 2015-11-13 [PMID: 26566726]
Golden D, Saria EA, Hansen MF. Regulation of Osteoblast Migration Involving Receptor Activator of Nuclear Factor (NF)-Kappa B (RANK) Signaling J. Cell. Physiol. 2015-04-20 [PMID: 25893522]
Qiao Hongwen, Zhu Lin, Lieberman Brian P et al. Synthesis and evaluation of novel tropane derivatives as potential PET imaging agents for the dopamine transporter. Bioorg Med Chem Lett. 2012-07-01 [PMID: 22658558]
Rajasekaran V. Tissue Transglutaminase (TG2) is a potential therapeutic target in the treatment of chemoresistant breast cancer. Breast Cancer Res 2012-01-10 [PMID: 22225906]
Li J, Liu Y, Zhang X. Murine coronavirus induces type I interferon in oligodendrocytes through recognition by RIG-I and MDA5. J Virol. 2010-07-01 [PMID: 20427526] (Mouse) Details: Functional Assay (TransAM ELISA): N20.1 cell line (mouse oligodendrocytes) infected with MHV-A59/GFP virus, Fig 7B, 7C, and 7D.Fig 7B, Readout assay: NFkB activation. Cells treated with NF-kB p65 inhibitory peptide (IMG-2003) showed significant inhibition		Mouse
Krupinski Elizabeth A. Virtual slide telepathology workstation of the future: lessons learned from teleradiology. Hum Pathol. 2009-08-01 [PMID: 19552939] (Human)		Human
Rebholz B, Kehe K, Ruzicka T, Rupec RA. Role of NF-kappaB/RelA and MAPK pathways in keratinocytes in response to sulfur mustard. J Invest Dermatol. 2008-07-01 [PMID: 18200059] (Mouse) Details: Mouse Keratinocytes, Fig. 6		Mouse
Takada Y, Singh S, Aggarwal BB. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004-04-09 [PMID: 14711835]
Show All 12 Publications. Collapse Publications.

Array NBP2-29321

• RelA/NFkB p65 Antibodies
• RelA/NFkB p65 Antibody Pack
• RelA/NFkB p65 ELISA Kits
• RelA/NFkB p65 Inhibitors
• RelA/NFkB p65 Kit
• RelA/NFkB p65 Lysate
• RelA/NFkB p65 Proteins