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RECQL5 Antibody

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Genetic Strategies: Western Blot: RECQL5 Antibody [NBP1-83438] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RECQL5 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: RECQL5 Antibody [NBP1-83438] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438] - Staining of human placenta shows no positivity in stromal cells as expected.
Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438] - Staining of human testis shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

RECQL5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ELEHETFRNAKVANLYKASVLKKVADIHRASKDGQPYDMGGSAKSCSAQAEPPEPNEYDIPPASHVYSLKPKRVGAGFPKGSCPFQTATELMETTRIREQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RECQL5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RECQL5 Protein (NBP1-83438PEP)
Publications
Read Publications using NBP1-83438.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RECQL5 Antibody

  • DNA helicase, RecQ-like type 5
  • EC 3.6.1
  • EC 3.6.4.12
  • FLJ90603
  • RecQ protein-like 5ATP-dependent DNA helicase Q5
  • RecQ5
  • RECQ5RecQ protein 5

Background

RECQL5 may have an important role in DNA metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RECQL5 Antibody (NBP1-83438)(2)

We have publications tested in 2 applications: IF/IHC, WB.


Filter By Application
IF/IHC
(1)
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RECQL5 Antibody (NBP1-83438) (0)

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Bioinformatics

Gene Symbol RECQL5