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Recombinant Rat TNF-alpha Protein, CF

 (67 Publications)
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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
510-RT-010
Carrier Free
510-RT-050/CF
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Recombinant Rat TNF-alpha Protein, CF Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-20 pg/mL.
Source
E. coli-derived rat TNF-alpha protein
Leu80-Leu235, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Tnf
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
510-RT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat TNF-alpha Protein, CF

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69%-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Shirai, T. et al. (1989) Agric. Biol. Chem. 53:1733.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Recombinant Rat
TNF-alpha Protein, CF
510-RT/CF
Recombinant Rat TNF-alpha Protein, CF
Species: Rt
Applications: Bioactivity

Publications for TNF-alpha (510-RT/CF)(67)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Primate - C. aethiops.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Binding Assay
(1)
Bioassay
(39)
Cell Culture
(3)
ELISA (Standard)
(3)
In Vivo
(24)
Western Blot
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(5)
Rat
(54)
Bovine
(2)
Primate - C. aethiops
(1)
All Species
Showing Publications 1 - 10 of 67. Show All 67 Publications.
Publications using 510-RT/CF Applications Species
M Silva Oliv, J Schira-Hei, L Reiche, S Han, VCM de Amorim, I Lewen, J Gruchot, P Göttle, R Akkermann, K Azim, P Küry Myelin repair is fostered by the corticosteroid medrysone specifically acting on astroglial subpopulations EBioMedicine, 2022-08-08;83(0):104204. 2022-08-08 [PMID: 35952494] (Bioassay, Mouse) Bioassay Mouse
I Rabinovich, A Blant, R Dhingra, LA Kirshenbau, MP Czubryt NF-kappaB p65 Attenuates Cardiomyocyte PGC-1alpha Expression in Hypoxia Cells, 2022-07-13;11(14):. 2022-07-13 [PMID: 35883637] (Bioassay, Primate - C. aethiops) Bioassay Primate - C. aethiops
A Badia-Sote, J de Vries, W Dykstra, LM Broersen, JM Verkuyl, AB Smit, MHG Verheijen High-Throughput Analysis of Astrocyte Cultures Shows Prevention of Reactive Astrogliosis by the Multi-Nutrient Combination Fortasyn Connect Cells, 2022-04-22;11(9):. 2022-04-22 [PMID: 35563732] (Bioassay, Mouse) Bioassay Mouse
A Singh, S Sateesh, OD Jones, WC Abraham Pathway-specific TNF-mediated metaplasticity in hippocampal area CA1 Scientific Reports, 2022-02-02;12(1):1746. 2022-02-02 [PMID: 35110639] (Bioassay, Rat) Bioassay Rat
JY Son, JS Ju, YM Kim, DK Ahn TNF-alpha-Mediated RIPK1 Pathway Participates in the Development of Trigeminal Neuropathic Pain in Rats International Journal of Molecular Sciences, 2022-01-03;23(1):. 2022-01-03 [PMID: 35008931] (Bioassay, Rat) Bioassay Rat
AA Candadai, F Liu, A Verma, MS Adil, M Alfarhan, SC Fagan, PR Somanath, SP Narayanan Neuroprotective Effects of Fingolimod in a Cellular Model of Optic Neuritis Cells, 2021-10-28;10(11):. 2021-10-28 [PMID: 34831161] (Bioassay, Rat) Bioassay Rat
PA Hernandez, TD Jacobsen, NO Chahine Actomyosin contractility confers mechanoprotection against TNFalpha-induced disruption of the intervertebral disc Sci Adv, 2020-08-19;6(34):eaba2368. 2020-08-19 [PMID: 32875103] (Bioassay, Bovine) Bioassay Bovine
X Ding, W Yang, Q Ren, J Hu, S Yang, W Han, J Wang, X Wang, H Wang Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 2020-06-16;17(1):190. 2020-06-16 [PMID: 32546235] (Bioassay, Rat) Bioassay Rat
JW Duncan, JP Granger, MJ Ryan, HA Drummond Interleukin-17 Reduces &amp;betaENaC via MAPK Signaling in Vascular Smooth Muscle Cells Int J Mol Sci, 2020-04-22;21(8):. 2020-04-22 [PMID: 32331392] (Cell Culture, Rat) Cell Culture Rat
S Zhao, J Jiang, Y Jing, W Liu, X Yang, X Hou, L Gao, L Wei The concentration of tumor necrosis factor-&amp;alpha determines its protective or damaging effect on liver injury by regulating Yap activity Cell Death Dis, 2020-01-27;11(1):70. 2020-01-27 [PMID: 31988281] (Cell Culture, In Vivo, Rat) Cell Culture, In Vivo Rat
Show All 67 Publications.

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FAQs for TNF-alpha (510-RT/CF). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
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Bioinformatics

Gene Symbol Tnf
Uniprot