Recombinant Rat IL-9 Protein, CF Summary
Details of Functionality |
Measured in a cell proliferation assay using TS1 mouse helper T cells. Uyttenhove, C. et al. (1988) Proc. Natl. Acad. Sci. 85:6934. The ED50 for this effect is 3-15 ng/mL. |
Source |
Mouse myeloma cell line, NS0-derived rat IL-9 protein Gln19-Ala144 |
Accession # |
|
N-terminal Sequence |
Gln19 |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<0.01 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
14.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
30-35 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Rat IL-9 Protein, CF
Background
Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL‑9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4
+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature rat IL-9 shares 58% and 74% amino acid sequence identity with human and mouse IL-9, respectively.
- Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
- Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
- Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
- Townsend, M.J. et al. (2000) Immunity 13:573.
- Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
- Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
- Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
- Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
- Tan, C. et al. (2010) J. Immunol. 185:6795.
- Jager, A. et al. (2009) J. Immunol. 183:7169.
- Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
- Lu, L.-F. et al. (2006) Nature 442:997.
- Stassen, M. et al. (2000) J. Immunol. 164:5549.
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