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Recombinant Mouse Oncostatin M (OSM) Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Oncostatin M (OSM) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.25‑1 ng/mL.
Source
E. coli-derived mouse Oncostatin M/OSM protein
Ala24-Arg206, with an N-terminal Met
Accession #
N-terminal Sequence
Ala26
Protein/Peptide Type
Recombinant Proteins
Gene
Osm
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
495-MO/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Oncostatin M (OSM) Protein, CF

  • MGC20461
  • oncostatin M
  • oncostatin-M
  • OSM

Background

Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF and EPO. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mOSM.

The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha . It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.

  1. Yoshimura, A. et al. (1996) The EMBO Journal 15:1055.
  2. Ray, P. et al. (1996) Endocrinology 137:1151.
  3. Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.

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Publications for Oncostatin M/OSM (495-MO/CF)(60)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(49)
Cell Culture
(3)
ELISA (Standard)
(1)
In Vivo
(7)
Western Blot
(2)
All Applications
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Mouse
(54)
Rat
(3)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using 495-MO/CF Applications Species
S Lee, R Karns, S Shin Mechanism of paracrine communications between hepatic progenitor cells and endothelial cells Cellular Signalling, 2022-08-30;100(0):110458. 2022-08-30 [PMID: 36055565] (Cell Culture, Mouse) Cell Culture Mouse
JL Bailey, H Hang, A Boudreau, CM Elks Oncostatin M Induces Lipolysis and Suppresses Insulin Response in 3T3-L1 Adipocytes International Journal of Molecular Sciences, 2022-04-23;23(9):. 2022-04-23 [PMID: 35563078] (Bioassay, Mouse) Bioassay Mouse
M Valussi, J Besser, K Wystub-Lis, S Zukunft, M Richter, T Kubin, T Boettger, T Braun Repression of Osmr and Fgfr1 by miR-1/133a prevents cardiomyocyte dedifferentiation and cell cycle entry in the adult heart Science Advances, 2021-10-13;7(42):eabi6648. 2021-10-13 [PMID: 34644107] (Bioassay, Mouse) Bioassay Mouse
PP van Krieke, TS Odermatt, M Borsigova, M Blüher, S Wueest, D Konrad Oncostatin M suppresses browning of white adipocytes via gp130-STAT3 signaling Molecular Metabolism, 2021-09-20;0(0):101341. 2021-09-20 [PMID: 34547509] (Bioassay, Mouse) Bioassay Mouse
K Anzai, K Tsuruya, K Ida, T Kagawa, Y Inagaki, A Kamiya Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts Scientific Reports, 2021-09-17;11(1):18551. 2021-09-17 [PMID: 34535735] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
N Tanimizu, N Ichinohe, Y Sasaki, T Itoh, R Sudo, T Yamaguchi, T Katsuda, T Ninomiya, T Tokino, T Ochiya, A Miyajima, T Mitaka Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo Nature Communications, 2021-06-07;12(1):3390. 2021-06-07 [PMID: 34099675] (Bioassay, Mouse) Bioassay Mouse
J Zeng, Y Jing, Q Wu, J Zeng, L Wei, J Liu Autophagy Is Required for Hepatic Differentiation of Hepatic Progenitor Cells via Wnt Signaling Pathway BioMed Research International, 2021-04-08;2021(0):6627506. 2021-04-08 [PMID: 33928152] (Bioassay, Rat) Bioassay Rat
K Kwiecien, P Brzoza, M Bak, P Majewski, I Skulimowsk, K Bednarczyk, J Cichy, M Kwitniewsk The methylation status of the chemerin promoter region located from -?252 to +?258�bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels Sci Rep, 2020-08-13;10(1):13702. 2020-08-13 [PMID: 32792625] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
S Alcalá, P Sancho, P Martinelli, D Navarro, C Pedrero, L Martín-Hij, S Valle, J Earl, M Rodríguez-, L Ruiz-Cañas, K Rojas, A Carrato, L García-Ber, MÁ Fernández-, PC Hermann, B Sainz ISG15 and ISGylation is required for pancreatic cancer stem cell mitophagy and metabolic plasticity Nat Commun, 2020-05-29;11(1):2682. 2020-05-29 [PMID: 32472071] (Bioassay, Mouse) Bioassay Mouse
J Lim, JH Choi, EM Park, YH Choi Interaction of promyelocytic leukemia/p53 affects signal transducer and activator of transcription-3 activity in response to oncostatin M Korean J. Physiol. Pharmacol., 2020-05-01;24(3):203-212. 2020-05-01 [PMID: 32395071] (Bioassay, Mouse) Bioassay Mouse
Show All 60 Publications.

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Bioinformatics

Gene Symbol Osm
Uniprot