Recombinant Mouse GDF-8 Propeptide Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1-3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF‑8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF‑8 propeptide functions as an inhibitor of mature GDF‑8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK‑4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Binding Assay, Bioassay, Control, ELISA Developmet, Enzyme Assay, Surface Plasmon Resonance.

Showing Publications 1 - 4 of 4.

Publications using 1539-PG/CF	Applications	Species
SB Lee, SK Park, YS Kim Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli PLoS ONE, 2017-04-03;12(4):e0174956. 2017-04-03 [PMID: 28369115] (Bioassay, Enzyme Assay, Human, Mouse)	Bioassay, Enzyme Assay	Human, Mouse
Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-07-02;381(4):1025-39. 2008-07-02 [PMID: 18621057] (ELISA Developmet, Surface Plasmon Resonance, Human)	ELISA Developmet, Surface Plasmon Resonance	Human
Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY Targeting of bone morphogenetic protein growth factor complexes to fibrillin. J. Biol. Chem., 2008-03-13;283(20):13874-88. 2008-03-13 [PMID: 18339631] (Binding Assay, Surface Plasmon Resonance, Human)	Binding Assay, Surface Plasmon Resonance	Human
Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition. J. Clin. Invest., 2006-10-12;116(11):2924-34. 2006-10-12 [PMID: 17039257] (Control, Mouse)	Control	Mouse

Array 1539-PG/CF

• GDF-8/Myostatin Antibodies
• GDF-8/Myostatin Lysate
• GDF-8/Myostatin ELISAs
• GDF-8/Myostatin Proteins and Enzymes