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Recombinant Mouse FGF acidic/FGF1 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Carrier Free
4686-FA-025/CF
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Recombinant Mouse FGF acidic/FGF1 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.05-0.25 ng/mL in the presence of 10 µg/mL heparin.
Source
E. coli-derived mouse FGF acidic/FGF1 protein
Phe16-Asp155, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
4686-FA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF acidic/FGF1 Protein

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Background

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Mouse FGF acidic shares 52% amino acid (aa) sequence identity with FGF basic and 15%-35% with other mouse FGFs. It shares 91%, 96%, 94%, and 100% aa sequence identity with bovine, human, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). The secreted FGF acidic is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulating FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10-12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

  1. Madiai, F. et al. (1996) Gene 179:231.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  11. Imamura, T. et al. (1990) Science 249:1567.
  12. Wesche, J. et al. (2005) Biochemistry 44:6071.
  13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  15. Bouleau, S. et al. (2005) Oncogene 24:7839.

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Publications for FGF acidic/FGF1 (4686-FA)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, IP, Stimulation.


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Bioassay
(5)
IP
(1)
Stimulation
(1)
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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using 4686-FA Applications Species
F Pei, L Ma, J Jing, J Feng, Y Yuan, T Guo, X Han, TV Ho, J Lei, J He, M Zhang, JF Chen, Y Chai Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis Nature Communications, 2023-01-20;14(1):344. 2023-01-20 [PMID: 36670126] (Bioassay, Mouse) Bioassay Mouse
K Ichikawa, S Watanabe M, Y Minoshima, J Matsui, Y Funahashi Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy Sci Rep, 2020-02-19;10(1):2939. 2020-02-19 [PMID: 32076044] (IP, Mouse) IP Mouse
J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment Sci Rep, 2019-06-19;9(1):8726. 2019-06-19 [PMID: 31217507] (Bioassay, Mouse) Bioassay Mouse
A Taguchi, R Nishinakam Higher-Order Kidney Organogenesis from Pluripotent Stem Cells Cell Stem Cell, 2017-11-09;0(0):. 2017-11-09 [PMID: 29129523] (Bioassay, Mouse) Bioassay Mouse
Gui T, Sun Y, Gai Z, Shimokado A, Muragaki Y, Zhou G The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling. Dev Biol, 2013-03-26;377(2):415-27. 2013-03-26 [PMID: 23537899] (Mouse) Mouse
Kharitonenkov , Alexei, Beals , John M, Micanovic , Radmila, Strifler , Beth A, Rathnachalam , Radhakri, Wroblewski , Victor J, Li , Shun, Koester , Anja, Ford , Amy M, Coskun , Tamer, Dunbar , James D, Cheng , Christin, Frye , Christop, Bumol , Thomas F, Moller , David E Rational design of a fibroblast growth factor 21-based clinical candidate, LY2405319. PLoS ONE, 2013-03-11;8(3):e58575. 2013-03-11 [PMID: 23536797] (Stimulation, Mouse) Stimulation Mouse
Tucker B, Anfinson K, Mullins R, Stone E, Young M Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med, 2012-12-27;2(1):16-24. 2012-12-27 [PMID: 23283489] (Bioassay, Mouse) Bioassay Mouse
Yamamoto S, Fukumoto E, Yoshizaki K, Iwamoto T, Yamada A, Tanaka K, Suzuki H, Aizawa S, Arakaki M, Yuasa K, Oka K, Chai Y, Nonaka K, Fukumoto S Platelet-derived growth factor receptor regulates salivary gland morphogenesis via fibroblast growth factor expression. J. Biol. Chem., 2008-06-17;283(34):23139-49. 2008-06-17 [PMID: 18559345] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Fgf1
Uniprot