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Recombinant Mouse CCL2/JE/MCP-1 Protein, CF

Name: Recombinant Mouse CCL2/JE/MCP-1 Protein, CF
Brand: Novus Biologicals
SKU: 479-JE/CF
Price: 271 USD

(92 Publications)

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Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE/CF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED50 for this effect is 2-10 ng/mL.

1 μg/lane of Recombinant Mouse CCL2/JE/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 8 kDa.

Product Details

Summary

Reactivity	MuSpecies Glossary
Applications	Bioactivity
Format	Carrier-Free

Order Details

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation 479-JE-010

Carrier Free 479-JE-050/CF

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Recombinant Mouse CCL2/JE/MCP-1 Protein, CF Summary

Details of Functionality	Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED₅₀ for this effect is 2-10 ng/mL.
Source	E. coli-derived mouse CCL2/JE/MCP-1 protein Gln24-Arg96
Accession #	Q5SVU3
N-terminal Sequence	Gln24
Protein/Peptide Type	Recombinant Proteins
Gene	Ccl2
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note	<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions

Bioactivity

Theoretical MW

8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Publications

Read Publications using
479-JE/CF in the following applications:

Binding Assay
1 publication
Bioassay
63 publications
Cell Culture
4 publications
ELISA (Standard)
6 publications
ELISA Capture
1 publication
In Vivo
18 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS.
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL2/JE/MCP-1 Protein, CF

C-C motif chemokine ligand 2
CCL2
GDCF-2
HC11
HSMCR30
MCAF
Mcp1
MCP-1
SCYA2
SMC-CF

Background

CCL2, also called monocyte chemotactic protein-1 (MCP-1) or JE, is a member of the C-C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Mouse CCL2 cDNA encodes a 148 amino acid (aa) precursor protein with a 23 aa signal peptide and a 125 aa mature protein (1). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding (3). Mouse CCL2 forms a broad band around 25 kDa on SDS-PAGE due to non-covalent dimerization and variable carbohydrate content (1). Mouse and rat express a form of CCL2 that is extended by 49 aa compared to other species. Mature mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively, over aa 24 ‑ 101. Human CCL2 can, however, induce a limited response in rodent cells, and mouse CCL2 has full activity on human cells (2, 4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4⁺ T cells (5). CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7). When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay.

Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA. 85:3738.
Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
Dean, R.A. et al. (2008) Blood. 112:3455.
Van Riper, G. et al. (1993) J. Exp. Med. 177:851.
Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
Daly, C. et al. (2003) Microcirculation 10:247.
Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.

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Recombinant Mouse
CCL2/JE/MCP-1 Protein ...

479-JE/CF

Species: Mu

Applications: Bioactivity

Publications for CCL2/JE/MCP-1 (479-JE/CF)(92)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Capture, In Vivo.

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Binding Assay

(1)

Bioassay

(63)

Cell Culture

(4)

ELISA (Standard)

(6)

ELISA Capture

(1)

In Vivo

(18)

All Applications

Filter By Species

Human

(5)

Mouse

(81)

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(1)

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Showing Publications 1 - 10 of 92. Show All 92 Publications.

Publications using 479-JE/CF	Applications	Species
Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH; Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation Mucosal immunology 2023-05-10 [PMID: 37172907] (Bioassay, Mouse)	Bioassay	Mouse
Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function PLoS ONE, 2023-01-20;18(1):e0280590. 2023-01-20 [PMID: 36662882] (Bioassay, Human)	Bioassay	Human
J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity Cell Reports, 2023-01-18;42(1):111991. 2023-01-18 [PMID: 36656709] (Bioassay, Mouse)	Bioassay	Mouse
HJ Choi, SJ Park, YN Choi, SD Kim, EB Kwag, SY Song, JH Park, JK Kim, C Seo, JJ Choi, HS Yoo Selective Immune Modulating Activities of Viscum album and Its Components; A Possibility of Therapeutics on Skin Rash Induced by EGFR Inhibitors Integrative cancer therapies, 2022-01-01;21(0):1534735422111. 2022-01-01 [PMID: 36154312] (Bioassay, Mouse)	Bioassay	Mouse
A Rajesh, S Droho, JA Lavine Macrophages in close proximity to the vitreoretinal interface are potential biomarkers of inflammation during retinal vascular disease Journal of Neuroinflammation, 2022-08-08;19(1):203. 2022-08-08 [PMID: 35941655] (In Vivo, Mouse)	In Vivo	Mouse
FH Brennan, Y Li, C Wang, A Ma, Q Guo, Y Li, N Pukos, WA Campbell, KG Witcher, Z Guan, KA Kigerl, JCE Hall, JP Godbout, AJ Fischer, DM McTigue, Z He, Q Ma, PG Popovich Microglia coordinate cellular interactions during spinal cord repair in mice Oncogene, 2022-07-14;13(1):4096. 2022-07-14 [PMID: 35835751] (In Vivo, Mouse)	In Vivo	Mouse
M Itakura, K Yamaguchi, R Kitazawa, SY Lim, Y Anan, J Yoshitake, T Shibata, L Negishi, H Sugawa, R Nagai, K Uchida Histone functions as a cell-surface receptor for AGEs Nature Communications, 2022-05-27;13(1):2974. 2022-05-27 [PMID: 35624109] (Bioassay, Mouse)	Bioassay	Mouse
L Ye, K Jin, Z Liao, Z Xiao, H Xu, X Lin, H Li, T Li, W Zhang, X Han, W Wang, H Gao, L Liu, W Wu, X Yu Hypoxia-reprogrammed regulatory group 2 innate lymphoid cells promote immunosuppression in pancreatic cancer EBioMedicine, 2022-04-25;79(0):104016. 2022-04-25 [PMID: 35483273] (Bioassay, Mouse)	Bioassay	Mouse
YJ Ahn, L Wang, S Tavakoli, HN Nguyen, JD Short, R Asmis Glutaredoxin 1 controls monocyte reprogramming during nutrient stress and protects mice against obesity and atherosclerosis in a sex-specific manner Nature Communications, 2022-02-10;13(1):790. 2022-02-10 [PMID: 35145079] (Bioassay, Mouse)	Bioassay	Mouse
YC Fan, KC Hsu, TE Lin, D Zechner, SP Hsu, YC Tsai Investigation of Anti-Tumor Effects of an MLK1 Inhibitor in Prostate and Pancreatic Cancers Biology, 2021-08-02;10(8):. 2021-08-02 [PMID: 34439974] (Bioassay, Human, Mouse)	Bioassay	Human, Mouse
LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Nature Communications, 2021-06-10;12(1):3543. 2021-06-10 [PMID: 34112803] (ELISA Capture, Mouse)	ELISA Capture	Mouse
S Ishihara, AM Obeidat, DL Wokosin, D Ren, RJ Miller, AM Malfait, RE Miller The role of intra-articular neuronal CCR2 receptors in knee joint pain associated with experimental osteoarthritis in mice Arthritis Research & Therapy, 2021-04-07;23(1):103. 2021-04-07 [PMID: 33827672] (Bioassay, Mouse)	Bioassay	Mouse
Z Maria, E Turner, A Agasing, G Kumar, RC Axtell Pertussis Toxin Inhibits Encephalitogenic T-Cell Infiltration and Promotes a B-Cell-Driven Disease during Th17-EAE International Journal of Molecular Sciences, 2021-03-13;22(6):. 2021-03-13 [PMID: 33805762] (Bioassay, Mouse)	Bioassay	Mouse
A Villarreal, MW Tiruneh, J Lee, CF Guerrero-J, J Kuhn, JA David, K Dammeyer, R Mc Kell, J Kwong, PS Rabbani, Q Nie, DJ Ceradini Keratinocyte-Macrophage Crosstalk by the Nrf2/Ccl2/EGF Signaling Axis Orchestrates Tissue Repair Cell Rep, 2020-11-24;33(8):108417. 2020-11-24 [PMID: 33238115] (In Vivo, Mouse)	In Vivo	Mouse
K Popiolek-B, A Ciechanows, K Ciapa?a, K Pawlik, M Oggioni, D Mercurio, MG De Simoni, J Mika -->The CCL2/CCL7/CCL12/CCR2 pathway is substantially and persistently upregulated in mice after traumatic brain injury, and CCL2 modulates the complement system in microglia Mol Cell Probes, 2020-11-04;54(0):101671. 2020-11-04 [PMID: 33160071] (Cell Culture, Mouse)	Cell Culture	Mouse
L Wang, S Wang, Y Shi, R Li, S Günther, YT Ong, M Potente, Z Yuan, E Liu, S Offermanns YAP and TAZ protect against white adipocyte cell death during obesity Nat Commun, 2020-10-28;11(1):5455. 2020-10-28 [PMID: 33116140] (Bioassay, Mouse)	Bioassay	Mouse
K Wang, Y Gu, Y Liao, S Bang, CR Donnelly, O Chen, X Tao, AJ Mirando, MJ Hilton, RR Ji PD-1 blockade inhibits osteoclast formation and murine bone cancer pain J. Clin. Invest., 2020-07-01;0(0):. 2020-07-01 [PMID: 32484460] (Bioassay, Mouse)	Bioassay	Mouse
M Jerebtsova, A Ahmad, N Kumari, O Rutagarama, S Nekhai Macrophage HIV-1 Gene Expression and Delay Resolution of Inflammation in HIV-Tg Mice Viruses, 2020-03-01;12(3):. 2020-03-01 [PMID: 32121564] (Cell Culture, Mouse)	Cell Culture	Mouse
M Jerebtsova, A Ahmad, X Niu, O Rutagarama, S Nekhai HIV-1 Transcription Inhibitor 1E7-03 Restores LPS-Induced Alteration of Lung Leukocytes' Infiltration Dynamics and Resolves Inflammation in HIV Transgenic Mice Viruses, 2020-02-12;12(2):. 2020-02-12 [PMID: 32059509] (Bioassay, Mouse)	Bioassay	Mouse
GB Kitchen, PS Cunningham, TM Poolman, M Iqbal, R Maidstone, M Baxter, J Bagnall, N Begley, B Saer, T Hussell, LC Matthews, DH Dockrell, HJ Durrington, JE Gibbs, JF Blaikley, AS Loudon, DW Ray The clock gene Bmal1 inhibits macrophage motility, phagocytosis, and impairs defense against pneumonia Proc. Natl. Acad. Sci. U.S.A., 2020-01-03;0(0):. 2020-01-03 [PMID: 31900362] (Cell Culture, Mouse)	Cell Culture	Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)	Binding Assay	Mouse
K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia Cytokine, 2019-04-16;119(0):202-213. 2019-04-16 [PMID: 31003094] (In Vivo, Mouse)	In Vivo	Mouse
M Qiu, K Huang, Y Liu, Y Yang, H Tang, X Liu, C Wang, H Chen, Y Xiong, J Zhang, J Yang Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis Mucosal Immunol, 2019-02-12;0(0):. 2019-02-12 [PMID: 30755716] (Bioassay, Mouse)	Bioassay	Mouse
Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells Cell Rep, 2019-02-05;26(6):1533-1543.e4. 2019-02-05 [PMID: 30726736] (Bioassay, Mouse)	Bioassay	Mouse
PLD Silva, F Lauretti-F, M Caldas de, SS Lima, AE Covarrubia, M De Franco, E Carvalho, PL Ho, RMA da Costa, EAL Martins, JB Da Silva Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines BMC Microbiol., 2019-01-07;19(1):4. 2019-01-07 [PMID: 30616505] (Bioassay, Mouse)	Bioassay	Mouse
T Kubota, M Inoue, N Kubota, I Takamoto, T Mineyama, K Iwayama, K Tokuyama, M Moroi, K Ueki, T Yamauchi, T Kadowaki Downregulation of macrophage Irs2 by hyperinsulinemia impairs IL-4-indeuced M2a-subtype macrophage activation in obesity Nat Commun, 2018-11-19;9(1):4863. 2018-11-19 [PMID: 30451856] (Bioassay, Mouse)	Bioassay	Mouse
S Lee, JS Kong, S You, HM Kwon, SA Yoo, CS Cho, WU Kim Transcription Factor NFAT5 Promotes Migration and Invasion of Rheumatoid Synoviocytes via Coagulation Factor III and CCL2 J. Immunol., 2018-05-23;0(0):. 2018-05-23 [PMID: 29794013] (Bioassay, Human)	Bioassay	Human
A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis J. Biol. Chem., 2018-04-17;0(0):. 2018-04-17 [PMID: 29666185] (Bioassay, Mouse)	Bioassay	Mouse
J Guo, X Qiu, L Zhang, R Wei Smurf1 regulates macrophage proliferation, apoptosis and migration via JNK and p38 MAPK signaling pathways Mol. Immunol., 2018-03-15;97(0):20-26. 2018-03-15 [PMID: 29550577] (Bioassay, Mouse)	Bioassay	Mouse
V Johnson, T Webb, A Norman, J Coy, J Kurihara, D Regan, S Dow Activated Mesenchymal Stem Cells Interact with Antibiotics and Host Innate Immune Responses to Control Chronic Bacterial Infections Sci Rep, 2017-08-29;7(1):9575. 2017-08-29 [PMID: 28851894] (Bioassay, Mouse)	Bioassay	Mouse
C Freitas, M Wittner, J Nguyen, V Rondeau, V Biajoux, ML Aknin, F Gaudin, S Beaussant-, Y Bertrand, C Bellanné-C, J Donadieu, F Bachelerie, M Espéli, A Dalloul, F Louache, K Balabanian Lymphoid differentiation of hematopoietic stem cells requires efficient Cxcr4 desensitization J. Exp. Med., 2017-05-26;0(0):. 2017-05-26 [PMID: 28550161] (Bioassay, Mouse)	Bioassay	Mouse
K Hosaka, K Rojas, HZ Fazal, MB Schneider, J Shores, V Federico, M McCord, L Lin, B Hoh Monocyte Chemotactic Protein-1-Interleukin-6-Osteopontin Pathway of Intra-Aneurysmal Tissue Healing Stroke, 2017-03-14;0(0):. 2017-03-14 [PMID: 28292871] (Bioassay, Mouse)	Bioassay	Mouse
Y Zhang, Y Wei, B Jiang, L Chen, H Bai, X Zhu, X Li, H Zhang, Q Yang, J Ma, Y Xu, J Ben, DC Christiani, Q Chen Scavenger receptor A1 prevents metastasis of non-small cell lung cancer via suppression of macrophage serum amyloid A1 Cancer Res, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202524] (Bioassay, Mouse)	Bioassay	Mouse
H Nakano, MR Lyons-Cohe, GS Whitehead, K Nakano, DN Cook Distinct functions of CXCR4, CCR2, and CX3CR1 direct dendritic cell precursors from the bone marrow to the lung J. Leukoc. Biol, 2017-02-01;0(0):. 2017-02-01 [PMID: 28148720] (Bioassay, Mouse)	Bioassay	Mouse
N Lavender, J Yang, SC Chen, J Sai, CA Johnson, P Owens, GD Ayers, A Richmond The Yin/Yan of CCL2: a minor role in neutrophil anti-tumor activity in vitro but a major role on the outgrowth of metastatic breast cancer lesions in the lung in vivo BMC Cancer, 2017-01-31;17(1):88. 2017-01-31 [PMID: 28143493] (Bioassay, Mouse)	Bioassay	Mouse
A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis Nat Commun, 2016-12-21;7(0):13781. 2016-12-21 [PMID: 28000790] (Bioassay, Mouse)	Bioassay	Mouse
Microbiota induces tonic CCL2 systemic levels that control pDC trafficking in steady state Mucosal Immunol, 2016-11-09;0(0):. 2016-11-09 [PMID: 27827374] (In Vivo, Mouse)	In Vivo	Mouse
Cong Lin High endogenous activated protein C levels attenuates bleomycin-induced pulmonary fibrosis J Cell Mol Med, 2016-06-14;0(0):. 2016-06-14 [PMID: 27295971] (Bioassay, Mouse)	Bioassay	Mouse
Platelets Guide Leukocytes to Their Sites of Extravasation PLoS Biol, 2016-05-06;14(5):e1002459. 2016-05-06 [PMID: 27152726] (In Vivo, Mouse)	In Vivo	Mouse
T Wang, Z Wang, P Yang, L Xia, M Zhou, S Wang, J Du, J Zhang PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice Cell Death Dis, 2016-04-07;7(0):e2176. 2016-04-07 [PMID: 27054331] (Bioassay, Mouse)	Bioassay	Mouse
Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease. J Clin Invest, 2015-06-29;125(8):3198-214. 2015-06-29 [PMID: 26121749] (Bioassay, Mouse)	Bioassay	Mouse
Costa M, de Negreiros C, Bornstein V, Valente R, Mengel J, Henriques M, Benjamim C, Penido C Murine IL-17+ Vgamma4 T lymphocytes accumulate in the lungs and play a protective role during severe sepsis. BMC Immunol, 2015-06-03;16(0):36. 2015-06-03 [PMID: 26037291] (Bioassay, Mouse)	Bioassay	Mouse
Eguchi A, Mulya A, Lazic M, Radhakrishnan D, Berk M, Povero D, Gornicka A, Feldstein A Microparticles release by adipocytes act as "find-me" signals to promote macrophage migration. PLoS ONE, 2015-04-07;10(4):e0123110. 2015-04-07 [PMID: 25849214] (Bioassay, Mouse)	Bioassay	Mouse
Viana I, de Almeida M, Lins M, dos Santos Reis M, de Araujo Vieira L, Smaniotto S Combined effect of insulin-like growth factor-1 and CC chemokine ligand 2 on angiogenic events in endothelial cells. PLoS ONE, 2015-04-01;10(4):e0121249. 2015-04-01 [PMID: 25830234] (Bioassay, Mouse)	Bioassay	Mouse
Morrison A, Yarovinsky T, Young B, Moraes F, Ross T, Ceneri N, Zhang J, Zhuang Z, Sinusas A, Pardi R, Schwartz M, Simons M, Bender J Chemokine-coupled beta2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis. J Exp Med, 2014-09-01;211(10):1957-68. 2014-09-01 [PMID: 25180062] (Bioassay, Mouse)	Bioassay	Mouse
Chen D, Goswami C, Burnett R, Anjanappa M, Bhat-Nakshatri P, Muller W, Nakshatri H Cancer affects microRNA expression, release, and function in cardiac and skeletal muscle. Cancer Res, 2014-06-30;74(16):4270-81. 2014-06-30 [PMID: 24980554] (Bioassay, Mouse)	Bioassay	Mouse
Menichella D, Abdelhak B, Ren D, Shum A, Frietag C, Miller R CXCR4 chemokine receptor signaling mediates pain in diabetic neuropathy. Mol Pain, 2014-06-25;10(0):42. 2014-06-25 [PMID: 24961298] (Bioassay, Mouse)	Bioassay	Mouse
Fleetwood A, Achuthan A, Schultz H, Nansen A, Almholt K, Usher P, Hamilton J Urokinase plasminogen activator is a central regulator of macrophage three-dimensional invasion, matrix degradation, and adhesion. J Immunol, 2014-03-10;192(8):3540-7. 2014-03-10 [PMID: 24616477] (Bioassay, Mouse)	Bioassay	Mouse
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014-03-02;20(4):377-84. 2014-03-02 [PMID: 24584118] (Bioassay, Mouse)	Bioassay	Mouse
Sato S, Zhang X The Friend leukaemia virus integration 1 (Fli-1) transcription factor affects lupus nephritis development by regulating inflammatory cell infiltration into the kidney. Clin Exp Immunol, 2014-07-01;177(1):102-9. 2014-07-01 [PMID: 24580413] (Bioassay, Mouse)	Bioassay	Mouse
Kallis , Yiannis, Scotton , Christop, Mackinnon , Alison C, Goldin , Robert D, Wright , Nicholas, Iredale , John P, Chambers , Rachel C, Forbes , Stuart J Proteinase activated receptor 1 mediated fibrosis in a mouse model of liver injury: a role for bone marrow derived macrophages. PLoS ONE, 2014-01-27;9(1):e86241. 2014-01-27 [PMID: 24475094] (Bioassay, Mouse)	Bioassay	Mouse
Bowcutt , R, Bramhall , M, Logunova , L, Wilson , J, Booth , C, Carding , S R, Grencis , R, Cruickshank , S A role for the pattern recognition receptor Nod2 in promoting recruitment of CD103+ dendritic cells to the colon in response to Trichuris muris infection. Mucosal Immunol, 2014-01-22;7(5):1094-105. 2014-01-22 [PMID: 24448097] (Bioassay, Mouse)	Bioassay	Mouse
Suga H, Rennert R, Rodrigues M, Sorkin M, Glotzbach J, Januszyk M, Fujiwara T, Longaker M, Gurtner G Tracking the elusive fibrocyte: identification and characterization of collagen-producing hematopoietic lineage cells during murine wound healing. Stem Cells, 2014-05-01;32(5):1347-60. 2014-05-01 [PMID: 24446236] (Bioassay, Mouse)	Bioassay	Mouse
Temme S, Jacoby C, Ding Z, Bonner F, Borg N, Schrader J, Flogel U Technical advance: monitoring the trafficking of neutrophil granulocytes and monocytes during the course of tissue inflammation by noninvasive 19F MRI. J Leukoc Biol, 2013-12-06;95(4):689-97. 2013-12-06 [PMID: 24319285] (In Vivo, Mouse)	In Vivo	Mouse
Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression. J Immunol, 2013-12-04;192(1):407-17. 2013-12-04 [PMID: 24307731] (Cell Culture, Mouse)	Cell Culture	Mouse
Fan Q, Gu D, Liu H, Yang L, Zhang X, Yoder M, Kaplan M, Xie J Defective TGF-beta signaling in bone marrow-derived cells prevents hedgehog-induced skin tumors. Cancer Res, 2013-11-26;74(2):471-83. 2013-11-26 [PMID: 24282281] (Bioassay, Mouse)	Bioassay	Mouse
Dong B, Zhang S, Gao W, Su H, Chen J, Jin F, Bhargava A, Chen X, Jorgensen L, Alberts A, Zhang J, Siminovitch K Mammalian diaphanous-related formin 1 regulates GSK3beta-dependent microtubule dynamics required for T cell migratory polarization. PLoS ONE, 2013-11-18;8(11):e80500. 2013-11-18 [PMID: 24260404] (Bioassay, Mouse)	Bioassay	Mouse
Zhu , Xudong, Zong , Guijuan, Zhu , Liu, Jiang , Yuchen, Ma , Ke, Zhang , Hanwen, Zhang , Yan, Bai , Hui, Yang , Qing, Ben , Jingjing, Li , Xiaoyu, Xu , Yong, Chen , Qi Deletion of class A scavenger receptor deteriorates obesity-induced insulin resistance in adipose tissue. Diabetes, 2013-10-29;63(2):562-77. 2013-10-29 [PMID: 24170693] (Bioassay, Mouse)	Bioassay	Mouse
Wanschel , Amarylis, Seibert , Tara, Hewing , Bernd, Ramkhelawon , Bhama, Ray , Tathagat, van Gils , Janine M, Rayner , Katey J, Feig , Jonathan, O'Brien , Edward R, Fisher , Edward A, Moore , Kathryn Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):886-93. 2013-02-21 [PMID: 23430613] (Bioassay, Mouse)	Bioassay	Mouse
Kim HS, Go H, Akira S, Chung DH TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice. J. Immunol., 2011-09-19;187(8):4007-17. 2011-09-19 [PMID: 21930967] (In Vivo, Mouse)	In Vivo	Mouse
Guerrot D, Kerroch M, Placier S, Vandermeersch S, Trivin C, Mael-Ainin M, Chatziantoniou C, Dussaule JC Discoidin domain receptor 1 is a major mediator of inflammation and fibrosis in obstructive nephropathy. Am. J. Pathol., 2011-05-13;179(1):83-91. 2011-05-13 [PMID: 21640971] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice. J. Leukoc. Biol., 2010-11-02;89(2):269-82. 2010-11-02 [PMID: 21048214] (Bioassay, Mouse)	Bioassay	Mouse
Koth LL, Cambier CJ, Ellwanger A DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2. J. Immunol., 2010-04-26;184(11):6522-8. 2010-04-26 [PMID: 20421649] (Bioassay, Mouse)	Bioassay	Mouse
Tateya S, Tamori Y, Kawaguchi T, Kanda H, Kasuga M An increase in the circulating concentration of monocyte chemoattractant protein-1 elicits systemic insulin resistance irrespective of adipose tissue inflammation in mice. Endocrinology, 2010-01-07;151(3):971-9. 2010-01-07 [PMID: 20056828] (In Vivo, Mouse)	In Vivo	Mouse
Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression. Int. J. Cancer, 2009-09-15;125(6):1276-84. 2009-09-15 [PMID: 19479998] (Bioassay, Mouse)	Bioassay	Mouse
Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice. J. Hepatol., 2009-04-22;51(1):55-66. 2009-04-22 [PMID: 19443073] (Bioassay, Mouse)	Bioassay	Mouse
Gao YJ, Zhang L, Samad OA, Suter MR, Yasuhiko K, Xu ZZ, Park JY, Lind AL, Ma Q, Ji RR JNK-induced MCP-1 production in spinal cord astrocytes contributes to central sensitization and neuropathic pain. J. Neurosci., 2009-04-01;29(13):4096-108. 2009-04-01 [PMID: 19339605] (In Vivo, Mouse)	In Vivo	Mouse
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA (Standard))	ELISA (Standard)
Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Nature, 2007-12-06;450(7171):825-31. 2007-12-06 [PMID: 18064003] (Bioassay, Mouse)	Bioassay	Mouse
Penido C, Costa MF, Souza MC, Costa KA, Candea AL, Benjamim CF, Henriques MG Involvement of CC chemokines in gammadelta T lymphocyte trafficking during allergic inflammation: the role of CCL2/CCR2 pathway. Int. Immunol., 2007-12-04;20(1):129-39. 2007-12-04 [PMID: 18056919] (In Vivo, Mouse)	In Vivo	Mouse
Kang JH, Kim CS, Han IS, Kawada T, Yu R Capsaicin, a spicy component of hot peppers, modulates adipokine gene expression and protein release from obese-mouse adipose tissues and isolated adipocytes, and suppresses the inflammatory responses of adipose tissue macrophages. FEBS Lett., 2007-08-14;581(23):4389-96. 2007-08-14 [PMID: 17719033] (Bioassay, Mouse)	Bioassay	Mouse
Das R, Burke T, Plow EF Histone H2B as a functionally important plasminogen receptor on macrophages. Blood, 2007-08-09;110(10):3763-72. 2007-08-09 [PMID: 17690254] (Bioassay, Mouse)	Bioassay	Mouse
Hollingsworth JW, Li Z, Brass DM, Garantziotis S, Timberlake SH, Kim A, Hossain I, Savani RC, Schwartz DA CD44 regulates macrophage recruitment to the lung in lipopolysaccharide-induced airway disease. Am. J. Respir. Cell Mol. Biol., 2007-04-19;37(2):248-53. 2007-04-19 [PMID: 17446529] (Bioassay, Mouse)	Bioassay	Mouse
Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (Bioassay, Human)	Bioassay	Human
Yamauchi LM, Aliberti JC, Baruffi MD, Portela RW, Rossi MA, Gazzinelli RT, Mineo JR, Silva JS The binding of CCL2 to the surface of Trypanosoma cruzi induces chemo-attraction and morphogenesis. Microbes Infect., 2006-12-08;9(1):111-8. 2006-12-08 [PMID: 17194609] (In Vivo, Mouse)	In Vivo	Mouse
Wang H, Peters T, Kess D, Sindrilaru A, Oreshkova T, Van Rooijen N, Stratis A, Renkl AC, Sunderkotter C, Wlaschek M, Haase I, Scharffetter-Kochanek K Activated macrophages are essential in a murine model for T cell-mediated chronic psoriasiform skin inflammation. J. Clin. Invest., 2006-08-01;116(8):2105-14. 2006-08-01 [PMID: 16886059] (In Vivo, Mouse)	In Vivo	Mouse
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA (Standard))	ELISA (Standard)
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury. J. Immunol., 2006-07-01;177(1):612-20. 2006-07-01 [PMID: 16785559] (ELISA (Standard))	ELISA (Standard)
Rhodes KE, Raivich G, Fawcett JW The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 2006-04-21;140(1):87-100. 2006-04-21 [PMID: 16631314] (In Vivo, Rat)	In Vivo	Rat
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA (Standard))	ELISA (Standard)
Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA (Standard))	ELISA (Standard)
Sergejeva S, Ivanov S, Lotvall J, Linden A Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation. Am. J. Respir. Cell Mol. Biol., 2005-05-18;33(3):248-53. 2005-05-18 [PMID: 15901616] (Bioassay, Mouse)	Bioassay	Mouse
Gonzalez de Aguilar JL, Dupuis L, Larmet Y Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons. Neurobiol. Dis., 2005-06-01;19(1):129-41. 2005-06-01 [PMID: 15837568] (Bioassay, Mouse)	Bioassay	Mouse
Perrin FE, Lacroix S, Aviles-Trigueros M, David S Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration. Brain, 2005-02-02;128(0):854-66. 2005-02-02 [PMID: 15689362] (In Vivo, Mouse)	In Vivo	Mouse
Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)	Bioassay	Mouse
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA (Standard))	ELISA (Standard)
Russell TD, Yan Q, Fan G, Khalifah AP, Bishop DK, Brody SL, Walter MJ IL-12 p40 homodimer-dependent macrophage chemotaxis and respiratory viral inflammation are mediated through IL-12 receptor beta 1. J. Immunol., 2003-12-15;171(12):6866-74. 2003-12-15 [PMID: 14662893] (Bioassay, Mouse)	Bioassay	Mouse
Penido C, Vieira-de-Abreu A, Bozza MT, Castro-Faria-Neto HC, Bozza PT Role of monocyte chemotactic protein-1/CC chemokine ligand 2 on gamma delta T lymphocyte trafficking during inflammation induced by lipopolysaccharide or Mycobacterium bovis bacille Calmette-Guerin. J. Immunol., 2003-12-15;171(12):6788-94. 2003-12-15 [PMID: 14662884] (In Vivo, Mouse)	In Vivo	Mouse
Bruemmer D, Collins AR, Noh G, Wang W, Territo M, Arias-Magallona S, Fishbein MC, Blaschke F, Kintscher U, Graf K, Law RE, Hsueh WA Angiotensin II-accelerated atherosclerosis and aneurysm formation is attenuated in osteopontin-deficient mice. J. Clin. Invest., 2003-11-01;112(9):1318-31. 2003-11-01 [PMID: 14597759] (Bioassay, Mouse)	Bioassay	Mouse
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol., 2003-02-01;170(3):1452-61. 2003-02-01 [PMID: 12538707] (Bioassay, Human)	Bioassay	Human
Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas. Immunol. Lett., 2002-06-03;82(1):111-21. 2002-06-03 [PMID: 12008042] (Bioassay, Mouse)	Bioassay	Mouse
Maus UA, Koay MA, Delbeck T, Mack M, Ermert M, Ermert L, Blackwell TS, Christman JW, Schlondorff D, Seeger W, Lohmeyer J Role of resident alveolar macrophages in leukocyte traffic into the alveolar air space of intact mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-06-01;282(6):L1245-52. 2002-06-01 [PMID: 12003780] (In Vivo, Mouse)	In Vivo	Mouse
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Gene Symbol	Ccl2
Uniprot	Q5SVU3()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Recombinant Mouse CCL2/JE/MCP-1 Protein, CF

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