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Recombinant Mouse CCL11/Eotaxin Protein

 (15 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
420-ME-020/CF
Carrier Free
420-ME-100/CF
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Recombinant Mouse CCL11/Eotaxin Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1‑5 ng/mL.
Source
E. coli-derived mouse CCL11/Eotaxin protein
His24-Pro97
Accession #
N-terminal Sequence
His24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl11
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
420-ME in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL11/Eotaxin Protein

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Mouse CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high amino acid sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma . In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor.

  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

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Publications for CCL11/Eotaxin (420-ME)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(6)
ELISA (Standard)
(6)
In Vivo
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 420-ME Applications Species
G Scabia, G Testa, M Scali, S Del Turco, G Desiato, N Berardi, A Sale, M Matteoli, L Maffei, M Maffei, M Mainardi, “Train the Reduced ccl11/eotaxin mediates the beneficial effects of environmental stimulation on the aged hippocampus Brain, Behavior, and Immunity, 2021-08-19;98(0):234-244. 2021-08-19 [PMID: 34418501] (Bioassay, Western Blot, Mouse) Bioassay, Western Blot Mouse
F Boscolo Se, D Fox, A Sacco Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy Cell Rep, 2019-01-15;26(3):689-701.e6. 2019-01-15 [PMID: 30650360] (Bioassay, Mouse) Bioassay Mouse
E Kindstedt, CK Holm, R Sulniute, I Martinez-C, R Lundmark, P Lundberg CCL11, a novel mediator of inflammatory bone resorption Sci Rep, 2017-07-13;7(1):5334. 2017-07-13 [PMID: 28706221] (Bioassay, Mouse) Bioassay Mouse
Erickson , Michelle, Morofuji , Yoichi, Owen , Joshua B, Banks , William Rapid transport of CCL11 across the blood-brain barrier: regional variation and importance of blood cells. J Pharmacol Exp Ther, 2014-04-04;349(3):497-507. 2014-04-04 [PMID: 24706984] (In Vivo, Mouse) In Vivo Mouse
Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Nature, 2011-08-31;477(7362):90-4. 2011-08-31 [PMID: 21886162] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
Nel HJ, Hams E, Saunders SP Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice. J. Immunol., 2011-03-11;186(8):4631-9. 2011-03-11 [PMID: 21398616] (In Vivo, Mouse) In Vivo Mouse
Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA (Standard)) ELISA (Standard)
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA (Standard)) ELISA (Standard)
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA (Standard)) ELISA (Standard)
De Lucca GV, Kim UT, Vargo BJ, Duncia JV, Santella JB, Gardner DS, Zheng C, Liauw A, Wang Z, Emmett G, Wacker DA, Welch PK, Covington M, Stowell NC, Wadman EA, Das AM, Davies P, Yeleswaram S, Graden DM, Solomon KA, Newton RC, Trainor GL, Decicco CP, Ko SS Discovery of CC chemokine receptor-3 (CCR3) antagonists with picomolar potency. J. Med. Chem., 2005-03-24;48(6):2194-211. 2005-03-24 [PMID: 15771462] (In Vivo, Mouse) In Vivo Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol Ccl11
Uniprot