Home » CCL1/I-309/TCA-3 » CCL1/I-309/TCA-3 Proteins » Recombinant Mouse CCL1/TCA-3 Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Mouse CCL1/TCA-3 Protein

 (8 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for CCL1/I-309/TCA-3 (845-TC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
845-TC-025/CF
Carrier Free
845-TC-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Mouse CCL1/TCA-3 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR8. The ED50 for this effect is 0.5-2.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CCL1/I-309/TCA-3 protein
Lys24-Cys92
Accession #
N-terminal Sequence
Lys24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13-16 kDa, reducing conditions
Publications
Read Publications using
845-TC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL1/TCA-3 Protein

  • C-C motif chemokine 1
  • CCL1
  • chemokine (C-C motif) ligand 1
  • I-309
  • inflammatory cytokine I-309
  • P500
  • SCYA1
  • SCYA1Small-inducible cytokine A1
  • SCYA2
  • SISe
  • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
  • T lymphocyte-secreted protein I-309
  • TCA3
  • TCA-3

Background

Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA-3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha , MIP-1 beta and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T-cells.

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.

Customers Who Viewed This Item Also Viewed...

CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(251 Publications)
Species: Hu
Applications: ELISA
CCR8 Antibody
(191704) [Unconjugate
MAB1429
Flow Cytometry CCR8 Antibody (191704) [Unconjugated]
Neutralization CCR8 Antibody (191704) [Unconjugated]
(3 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, Neut
CCL5/RANTES [HRP]
DRN00B
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
N/A CCL5/RANTES [HRP]
(100 Publications)
Species: Hu
Applications: ELISA
CCL17/TARC [HRP]
MCC170
N/A CCL17/TARC [HRP]
N/A CCL17/TARC [HRP]
(45 Publications)
Species: Mu
Applications: ELISA
CCL22/MDC [HRP]
DMD00
N/A CCL22/MDC [HRP]
N/A CCL22/MDC [HRP]
(31 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(807 Publications)
Species: Hu
Applications: BA
CXCL10/IP-10/CRG-2
[HRP]
DIP100
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
N/A CXCL10/IP-10/CRG-2 [HRP]
(152 Publications)
Species: Hu
Applications: ELISA
IL-4 [Unconjugated]
6507-IL/CF
Bioactivity IL-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CCL7/MCP-3/MARC
Antibody [Unconjugate
AF-456-NA
Neutralization CCL7/MCP-3/MARC Antibody [Unconjugated]
(15 Publications)
Species: Mu
Applications: Neut, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]
Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]
Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]
(1 Review)
(180 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(290 Publications)
Species: Mu
Applications: ELISA
IFN-gamma
[Unconjugated]
285-IF
Bioactivity IFN-gamma [Unconjugated]
SDS-PAGE IFN-gamma [Unconjugated]
SEC-MALS IFN-gamma [Unconjugated]
(2 Reviews)
(445 Publications)
Species: Hu
Applications: BA
CCL3/MIP-1 alpha
[HRP]
MMA00
N/A CCL3/MIP-1 alpha [HRP]
N/A CCL3/MIP-1 alpha [HRP]
(77 Publications)
Species: Mu
Applications: ELISA
CCL11/Eotaxin [HRP]
MME00
N/A CCL11/Eotaxin [HRP]
N/A CCL11/Eotaxin [HRP]
(57 Publications)
Species: Mu
Applications: ELISA
CCL4/MIP-1 beta
[HRP]
DMB00
N/A CCL4/MIP-1 beta [HRP]
N/A CCL4/MIP-1 beta [HRP]
N/A CCL4/MIP-1 beta [HRP]
(50 Publications)
Species: Hu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Nocturnin Antibody
NBP2-37856
Immunohistochemistry-Paraffin Nocturnin Antibody
Immunocytochemistry/ Immunofluorescence Nocturnin Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
CCR4 Antibody
(205410) [Unconjugate
MAB1567
Flow Cytometry CCR4 Antibody (205410) [Unconjugated]
(28 Publications)
Species: Hu
Applications: CyTOF-reported, Flow

Publications for CCL1/I-309/TCA-3 (845-TC)(8)

We have publications tested in 2 confirmed species: Mouse, Virus.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Standard).


Filter By Application
Bioassay
(5)
Cell Culture
(1)
ELISA (Standard)
(2)
All Applications
Filter By Species
Mouse
(5)
Virus
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 845-TC Applications Species
NS Haque, A Tuteja, N Haque CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells PLoS ONE, 2019-07-17;14(7):e0218944. 2019-07-17 [PMID: 31314754] (Cell Culture, Mouse) Cell Culture Mouse
Y Xu, X Dong, P Qi, Y Ye, W Shen, L Leng, L Wang, X Li, X Luo, Y Chen, P Sun, R Xiang, N Li Sox2 Communicates With Tregs Through CCL1 To Promote The Stemness Property Of Breast Cancer Cells Stem Cells, 2017-12-01;0(0):. 2017-12-01 [PMID: 29044882] (Bioassay, Mouse) Bioassay Mouse
SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin J. Immunol, 2016-02-05;196(6):2514-25. 2016-02-05 [PMID: 26851219] (Bioassay, Mouse) Bioassay Mouse
Wu Z, MacNeil A, Junkins R, Li B, Berman J, Lin T Mast cell FcepsilonRI-induced early growth response 2 regulates CC chemokine ligand 1-dependent CD4+ T cell migration. J Immunol, 2013-03-27;190(9):4500-7. 2013-03-27 [PMID: 23536637] (Bioassay, Mouse) Bioassay Mouse
Islam SA, Chang DS, Colvin RA Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011-01-09;12(2):167-77. 2011-01-09 [PMID: 21217759] (Bioassay, Mouse) Bioassay Mouse
Kearley J, Buckland KF, Mathie SA, Lloyd CM Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway. Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81. 2009-01-29 [PMID: 19179489] (ELISA (Standard)) ELISA (Standard)
Kearley J, Robinson DS, Lloyd CM CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling. J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6. 2008-07-30 [PMID: 18672278] (ELISA (Standard)) ELISA (Standard)
Najarro P, Lee HJ, Fox J, Pease J, Smith GL Yaba-like disease virus protein 7L is a cell-surface receptor for chemokine CCL1. J. Gen. Virol., 2003-12-01;84(0):3325-36. 2003-12-01 [PMID: 14645913] (Bioassay, Virus) Bioassay Virus

Reviews for CCL1/I-309/TCA-3 (845-TC) (0)

There are no reviews for CCL1/I-309/TCA-3 (845-TC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CCL1/I-309/TCA-3 (845-TC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CCL1/I-309/TCA-3 Products

Blogs on CCL1/I-309/TCA-3

There are no specific blogs for CCL1/I-309/TCA-3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse CCL1/TCA-3 Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol Ccl1
Uniprot