Recombinant Mouse Brevican Protein, CF Summary
Details of Functionality |
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Brevican is coated at 3 μg/mL (100 μL/well), the concentration of biotinylated hyaluronan that produces 50% of the optimal binding response is found to be approximately 1-6 ng/mL. |
Source |
Mouse myeloma cell line, NS0-derived mouse Brevican protein Met1-Leu883, with a C-terminal 6-His tag |
Accession # |
|
N-terminal Sequence |
Asp23 |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
Bcan |
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<1.0 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
94.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
130-145 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 100 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Brevican Protein, CF
Background
Brevican, also called BEHAB, is a member of the the lectican family of proteoglycans that share a common domain structure (1). It is restricted to the CNS and associates with perineuronal nets and astrocytes (2 ‑ 5). Brevican contains an Ig-like V-set domain, two link domains, a Glu-rich region, a central region with glycosaminoglycan (GAG) modifications, an EGF-like domain, a C-type lectin domain, and a C-terminal Sushi/CRP-like domain (6). Brevican consists of a 145 kDa core protein with up to approximately 100 kDa of attached chondroitin sulfate but not heparan sulfate chains (3, 7). Alternate splicing generates a GPI-anchored isoform that lacks the EGF-like, lectin, and Sushi domains (4, 6). Brevican is cleaved by multiple proteases and exists in a number of distinct fragments (8, 9). An under-glycosylated form of Brevican accumulates in highly aggressive glioma, Alzheimer’s-like brain, and following brain injury (10 - 12). Levels of an ADAMTS-generated 55 kDa N-terminal fragment accumulate during remodeling after excitotoxic injury, and Brevican cleavage is required for its ability to promote glioma adhesion and motility (13 ‑ 15). In contrast, Brevican cleavage is inhibited in mouse brain with amyloid beta deposits (10). Mouse Brevican shares 80%, 81%, and 95% aa sequence identity with bovine, human, and rat Brevican, respectively. Within the Ig-like and two link domains, mouse Brevican shares 47% - 51% aa sequence identity with mouse Aggrecan, Neurocan, and Versican.
- Maeda, N. et al. (2010) Front. Biosci. 15:626.
- Jaworski, D.M. et al. (1994) J. Cell Biol. 125:495.
- Yamada, H. et al. (1997) J. Neurosci. 17:7784.
- Seidenbecher, C.I. et al. (2002) J. Neurochem. 83:738.
- Deepa, S.S. et al. (2006) J. Biol. Chem. 281:17789.
- Rauch, U. et al. (1997) Genomics 44:15.
- Seidenbecher, C.I. et al. (1995) J. Biol. Chem. 270:27206.
- Matthews, R.T. et al. (2000) J. Biol. Chem. 275:22695.
- Nakamura, H. et al. (2000) J. Biol. Chem. 275:38885.
- Ajmo, J.M. et al. (2010) J. Neurochem. 113:784.
- Viapiano, M.S. et al. (2005) Canc. Res. 65:6726.
- Viapiano, M.S. et al. (2003) J. Biol. Chem. 278:33239.
- Hu, B. et al. (2008) J. Biol. Chem. 283:24848.
- Mayer, J. et al. (2005) BMC Neurosci. 6:52.
- Yuan, W. et al. (2002) Neuroscience 114:1091.
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