Recombinant Mouse Angiopoietin-2 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature mouse Ang-2 is 478 amino acids (aa) in length. It contains one coiled‑coil domain (aa 159‑256) that mediates multimerization, and a C‑terminal fibrinogen-like domain (aa 275‑495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang‑2 is 65‑66 kDa in size. Under non‑reducing conditions, both natural and recombinant Ang‑2 form 140 kDa dimers, 200 kDa trimers, and 250‑300 kDa tetramers and pentamers (3‑5). Mature mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1  3, 6). Both Ang‑2 and the related Angiopoietin‑1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang-1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang‑2 becomes as a Tie-2 agonist (3, 7‑10). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (11, 12). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (7, 12, 13). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang‑1 during lymphatic vessel development (6, 14). It mediates the up‑regulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (15). Ang-2 is up‑regulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (16‑19). Its direct interaction with Integrins promotes tumor cell invasion (20, 21). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (19).

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.

Showing Publications 1 - 7 of 7.

Publications using 7186-AN/CF	Applications	Species
JH Bae, MJ Yang, SH Jeong, J Kim, SP Hong, JW Kim, YH Kim, GY Koh Gatekeeping role of Nf2/Merlin in vascular tip EC induction through suppression of VEGFR2 internalization Science Advances, 2022-06-10;8(23):eabn2611. 2022-06-10 [PMID: 35687678] (Bioassay, Mouse)	Bioassay	Mouse
R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner Cell Reports, 2021-08-17;36(7):109522. 2021-08-17 [PMID: 34407407] (Bioassay, Mouse)	Bioassay	Mouse
C Helaine, AE Ferré, MM Leblond, EA Pérès, M Bernaudin, S Valable, E Petit Angiopoietin-2 Combined with Radiochemotherapy Impedes Glioblastoma Recurrence by Acting in an Autocrine and Paracrine Manner: A Preclinical Study Cancers, 2020-11-30;12(12):. 2020-11-30 [PMID: 33266255] (Bioassay, Mouse)	Bioassay	Mouse
S Gadomski, SK Singh, S Singh, T Sarkar, KD Klarmann, M Berenschot, S Seaman, B Jakubison, KO Gudmundsso, S Lockett, JR Keller Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration Cell Rep, 2020-04-28;31(4):107572. 2020-04-28 [PMID: 32348770] (Bioassay, Mouse)	Bioassay	Mouse
AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res., 2019-06-12;0(0):. 2019-06-12 [PMID: 31189647] (Bioassay, In Vivo, Transgenic Mouse)	Bioassay, In Vivo	Transgenic Mouse
HS Kong, J Lee, HW Youm, SK Kim, JR Lee, CS Suh, SH Kim Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice PLoS ONE, 2017-09-15;12(9):e0184546. 2017-09-15 [PMID: 28915249] (In Vivo, Mouse)	In Vivo	Mouse
Effects of Angiopoietin-2 on Transplanted Mouse Ovarian Tissue PLoS ONE, 2016-11-21;11(11):e0166782. 2016-11-21 [PMID: 27870915] (In Vivo, Mouse)	In Vivo	Mouse

Array 7186-AN/CF

• Angiopoietin-2 Antibodies
• Angiopoietin-2 Antibody Pair
• Angiopoietin-2 Lysate
• Angiopoietin-2 Protein
• Angiopoietin-2 Proteins and Enzymes
• Angiopoietin-2 ELISAs