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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

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Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to ...read more
Recombinant Human TNF‑ alpha (HEK293-expressed) (Catalog # 10291-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect ...read more
2 μg/lane of Recombinant Human TNF-alpha (HEK293-expressed) (Catalog # 10291-TA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
10291-TA-050
Carrier Free
10291-TA-100
Carrier Free
10291-TA-250
Carrier Free
10291-TA-500
Carrier Free
10291-TA-01M
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Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 20-100 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived human TNF-alpha protein
Val77-Leu233
Accession #
N-terminal Sequence
Val77
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14-18 kDa, under reducing conditions
Publications
Read Publications using
10291-TA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Recombinant Human
TNF-alpha (HEK293-exp ...
10291-TA
Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF
Species: Hu
Applications: Bioactivity

Publications for TNF-alpha (10291-TA)(8)

We have publications tested in 3 confirmed species: Human, Mouse,  Human.

We have publications tested in 2 applications: Bioassay, Immunofluorescence.


Filter By Application
Bioassay
(7)
Immunofluorescence
(1)
All Applications
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Human
(6)
Mouse
(1)
 Human
(1)
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Showing Publications 1 - 8 of 8.
Publications using 10291-TA Applications Species
Hayderi, A;Zegeye, MM;Meydan, S;Sirsjö, A;Kumawat, AK;Ljungberg, LU; TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype International journal of molecular sciences 2024-08-09 [PMID: 39201392] (Bioassay, Human) Bioassay Human
Ulrich, A;Wu, Y;Draisma, H;Wharton, J;Swietlik, EM;Cebola, I;Vasilaki, E;Balkhiyarova, Z;Jarvelin, MR;Auvinen, J;Herzig, KH;Coghlan, JG;Lordan, J;Church, C;Howard, LS;Pepke-Zaba, J;Toshner, M;Wort, SJ;Kiely, DG;Condliffe, R;Lawrie, A;Gräf, S;Morrell, NW;Wilkins, MR;Prokopenko, I;Rhodes, CJ; Blood DNA methylation profiling identifies cathepsin Z dysregulation in pulmonary arterial hypertension Nature communications 2024-01-06 [PMID: 38184627] (Bioassay, Human) Bioassay Human
Mijit, M;Kpenu, E;Chowdhury, NN;Gampala, S;Wireman, R;Liu, S;Babb, O;Georgiadis, MM;Wan, J;Fishel, ML;Kelley, MR; In vitro and In vivo evidence demonstrating chronic absence of Ref-1 Cysteine 65 impacts Ref-1 folding configuration, redox signaling, proliferation and metastasis in pancreatic cancer Redox biology 2023-12-01 [PMID: 38056311] (Bioassay, Human) Bioassay Human
Barilli, A;Recchia Luciani, G;Visigalli, R;Sala, R;Soli, M;Dall'Asta, V;Rotoli, BM; Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology Biomedicines 2023-10-03 [PMID: 37893073] (Bioassay, Human) Bioassay Human
Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM; An integrated organoid omics map extends modeling potential of kidney disease Nature communications 2023-08-14 [PMID: 37580326] (Bioassay, Human) Bioassay Human
Isozaki, H;Sakhtemani, R;Abbasi, A;Nikpour, N;Stanzione, M;Oh, S;Langenbucher, A;Monroe, S;Su, W;Cabanos, HF;Siddiqui, FM;Phan, N;Jalili, P;Timonina, D;Bilton, S;Gomez-Caraballo, M;Archibald, HL;Nangia, V;Dionne, K;Riley, A;Lawlor, M;Banwait, MK;Cobb, RG;Zou, L;Dyson, NJ;Ott, CJ;Benes, C;Getz, G;Chan, CS;Shaw, AT;Gainor, JF;Lin, JJ;Sequist, LV;Piotrowska, Z;Yeap, BY;Engelman, JA;Lee, JJ;Maruvka, YE;Buisson, R;Lawrence, MS;Hata, AN; Therapy-induced APOBEC3A drives evolution of persistent cancer cells Nature 2023-07-05 [PMID: 37407818] (Immunofluorescence, Human) Immunofluorescence  Human
SW Wang, Q Zhang, D Lu, YC Fang, XC Yan, J Chen, ZK Xia, QT Yuan, LH Chen, YM Zhang, FJ Nan, X Xie GPR84 regulates pulmonary inflammation by modulating neutrophil functions Acta pharmacologica Sinica, 2023-04-04;0(0):1-11. 2023-04-04 [PMID: 37016043] (Bioassay, Mouse) Bioassay Mouse
S Salva, I Tiscornia, F Gutiérrez, S Alvarez, M Bollati-Fo Lactobacillus rhamnosus postbiotic-induced immunomodulation as safer alternative to the use of live bacteria Cytokine, 2021-07-10;146(0):155631. 2021-07-10 [PMID: 34252871] (Bioassay, Human) Bioassay Human

Reviews for TNF-alpha (10291-TA) (0)

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FAQs for TNF-alpha (10291-TA). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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