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Recombinant Human S100A8 Protein, CF

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Recombinant Human S100A8 (Catalog # 9876-S8) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 4-20 μg/mL.
Recombinant Human S100A8 (Catalog # 9876-S8) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 4-20 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human S100A8 Protein, CF Summary

Details of Functionality
Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 4-20 μg/mL.
Source
E. coli-derived human S100A8 protein
Met1-Glu93
Accession #
N-terminal Sequence
Met1
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Publications
Read Publications using
9876-S8 in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris and TCEP with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 150 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human S100A8 Protein, CF

  • 60B8AG
  • CAGACP-10
  • Calgranulin A
  • calgranulin-A
  • Calprotectin L1L subunit
  • CFAG
  • CFAGL1Ag
  • CGLA
  • Cystic fibrosis antigen
  • Leukocyte L1 complex light chain
  • MA387
  • MIF
  • Migration inhibitory factor-related protein 8
  • MRP8
  • MRP-8
  • MRP8S100 calcium binding protein A8 (calgranulin A)
  • NIF
  • P8
  • protein S100-A8
  • S100 calcium binding protein A8
  • S100 calcium-binding protein A8 (calgranulin A)
  • S100 calcium-binding protein A8
  • S100A8
  • Urinary stone protein band A

Background

S100A8 (also known as MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca2+ binding proteins (1, 2). It is produced by neutrophils and monocytes, and forms Ca2+ dependent heterodimer/ heterotetramer complexes (termed calprotectin) with S100A9 in addition to forming homodimeric complexes. Like S100A9, S100A8 is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. In addition, S100A8 was shown to activate lung alveolar epithelial cells, osteoclasts, and chondrocytes in TLR4-dependent manner, inducing proinflammatory cytokines and chemokines including, IL-6, IL-8, KC, and MCP-1 (6-8). In osteoarthritic chondrocytes, S100A8 and A9 also promoted expression of matrix metalloproteinases (MMP-1, -3, -9, and -13) potentially affecting to cartilage breakdown (8). Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12-47 and aa 46-81) and one high-affinity Ca2+ binding site (aa 59-70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP‑2 after Asn21, it is unclear if human S100A8 is susceptible to the same cleavage. Full-length human S100A8 is 57% and 61% identical to mouse and rat S100A8, respectively (9).
  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893
  3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  4. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  5. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250
  6. Chakraborty D. et al. (2017) Front Immunol. 8:1493
  7. Grevers L.C. Arthritis Rheum. 63:1365
  8. Schelbergen R.F. Arthritis Rheum. 64:1477
  9. Odink, K. et al. (1987) Nature 330:80

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Publications for S100A8 (9876-S8)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: Bioassay.


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Reviews for S100A8 (9876-S8) (0)

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FAQs for S100A8 (9876-S8). (Showing 1 - 2 of 2 FAQs).

  1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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