Home » Pappalysin-2/PAPP-A2 » Pappalysin-2 Peptides and Proteins » Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF

 (4 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for Pappalysin-2/PAPP-A2 (1668-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave IGFBP-5. Cleavage of Recombinant Human IGFBP‑5 (Catalog # 875-B5) by Recombinant Human Pappalysin‑2/PAPP‑A2 is detectable by SDS-PAGE stained by Coomassie, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Pappalysin-2/PAPP-A2 protein
Ser234-Cys1396, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser234
Protein/Peptide Type
Recombinant Enzymes
Gene
PAPPA2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
168 kDa, reducing conditions
Publications
Read Publications using
1668-ZN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Assay Procedure
  • Assay Buffer: 50 mM Tris, pH 8.0
  • Recombinant Human Pappalysin-2/PAPP-A2 (rhPappalysin-2) (Catalog # 1668-ZN)
  • Recombinant Human IGFBP‑5 (rhIGFBP-5) (Catalog # 875-B5)
  • 15% SDS-PAGE gel
  • Reducing SDS-PAGE gel buffer
  • SDS-PAGE or Western Blot
  1. Dilute rhPappalysin-2 to 40 µg/mL in Assay Buffer.
  2. Dilute rhIGFBP-5 to 200 µg/mL in Assay Buffer (Note: Acetonitrile interferes with assay, dry down rhIGFBP-5 and reconstitute at 200 µg/mL with Assay Buffer if necessary).
  3. Combine equal volumes of 40 µg/mL rhPappalysin-2 and 200 µg/mL rhIGFBP-5 for a 1:23.5 ratio of enzyme to Substrate. Include two blank controls containing Assay Buffer in place of rhPappalysin-2.
  4. Incubate reaction vials and one control at 37 °C overnight. Keep the other control at -20 °C during the overnight incubation period.
  5. Combine 20 µL from each reaction vial with 20 µL reducing SDS-PAGE gel buffer.
  6. Analyze the cleavage by SDS PAGE (load 40 µL/lane) followed by protein staining and/or Western blot.
Per Lane:
  • rhPappalysin-2: 0.4 µg
  • rhIGFBP-5: 2.0 µg

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF

  • EC 3.4.24.-
  • PAPPA2
  • PAPP-A2
  • PAPP-A2pappalysin-2
  • pappalysin 2
  • Pappalysin2
  • Pappalysin-2
  • PAPPE
  • PAPP-E
  • PLAC3
  • placenta-specific 3
  • pregnancy-associated plasma preproprotein-A2
  • Pregnancy-associated plasma protein A2
  • Pregnancy-associated plasma protein E1

Background

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP‑E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5 under the conditions described in Activity Assay Protocol.

  1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
  2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Customers Who Viewed This Item Also Viewed...


Pappalysin-1/PAPP-A An ...
AF2487
Western Blot Pappalysin-1/PAPP-A Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: IP, WB
IGFBP-5 Antibody
AF578
Immunohistochemistry IGFBP-5 Antibody
Western Blot IGFBP-5 Antibody
Western Blot IGFBP-5 Antibody
(7 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, WB
IGFBP-4 Antibody
AF804
Neutralization IGFBP-4 Antibody
Immunohistochemistry IGFBP-4 Antibody
Immunohistochemistry IGFBP-4 Antibody
(4 Publications)
Species: Hu
Applications: IHC, Neut, WB
Siglec-6/CD327
Antibody (767329) [Un ...
MAB2859
Flow Cytometry Siglec-6/CD327 Antibody (767329) [Unconjugated]
Flow Cytometry Siglec-6/CD327 Antibody (767329) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
VEGFR1/Flt-1
Antibody [Unconjugate
AF321
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]
Western Blot VEGFR1/Flt-1 Antibody [Unconjugated]
(2 Reviews)
(73 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, IHC, WB
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA
alpha-Sarcoglycan
Antibody (JA51-81)
NBP2-67150
Immunohistochemistry-Paraffin alpha-Sarcoglycan Antibody (JA51-81)
Immunohistochemistry-Paraffin alpha-Sarcoglycan Antibody (JA51-81)
Immunohistochemistry-Frozen alpha-Sarcoglycan Antibody (JA51-81)
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, IP, WB
TAZ/WWTR1 Antibody
- BSA Free
NB110-58359
Immunoprecipitation TAZ/WWTR1 Antibody - BSA Free
Simple Western TAZ/WWTR1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence TAZ/WWTR1 Antibody - BSA Free
(1 Review)
(20 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, PLA, Simple Western, WB
GPHA2 Antibody
NBP3-17255
Western Blot GPHA2 Antibody
Immunocytochemistry/ Immunofluorescence GPHA2 Antibody
Species: Hu
Applications: ICC/IF, WB
ATP6V0A2 Antibody
NBP1-59069
Western Blot ATP6V0A2 Antibody
Western Blot ATP6V0A2 Antibody
Immunohistochemistry-Paraffin ATP6V0A2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Leptin/OB [HRP]
DLP00
N/A Leptin/OB [HRP]
N/A Leptin/OB [HRP]
(156 Publications)
Species: Hu
Applications: ELISA
NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Corticotropin
Releasing Factor Anti ...
H00001392-M02
Western Blot Corticotropin Releasing Factor Antibody (2B11)
Sandwich ELISA Corticotropin Releasing Factor Antibody (2B11)
(4 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
AP3B1 Antibody
NBP3-38363
Western Blot AP3B1 Antibody
Immunohistochemistry AP3B1 Antibody
Immunohistochemistry AP3B1 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
S100A8 Antibody
[Unconjugated]
AF3059
Immunocytochemistry S100A8 Antibody [Unconjugated]
Western Blot S100A8 Antibody [Unconjugated]
Simple Western S100A8 Antibody [Unconjugated]
(2 Reviews)
(27 Publications)
Species: Mu
Applications: ICC, Simple Western, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
Prolyl
Oligopeptidase/PREP A
NBP2-33950
Immunocytochemistry/ Immunofluorescence Prolyl Oligopeptidase/PREP Antibody
Immunohistochemistry-Paraffin Prolyl Oligopeptidase/PREP Antibody
Immunohistochemistry-Paraffin Prolyl Oligopeptidase/PREP Antibody
(3 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Recombinant Human
Pappalysin-2/PAPP-A2 ...
1668-ZN
Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF
Species: Hu
Applications: Enzyme Activity

Publications for Pappalysin-2/PAPP-A2 (1668-ZN)(4)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Antibody Absorption, Bioassay, ELISA (Standard), Western Blot.


Filter By Application
Antibody Absorption
(1)
Bioassay
(2)
ELISA (Standard)
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(3)
N/A
(1)
All Species

Reviews for Pappalysin-2/PAPP-A2 (1668-ZN) (0)

There are no reviews for Pappalysin-2/PAPP-A2 (1668-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Pappalysin-2/PAPP-A2 (1668-ZN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Pappalysin-2/PAPP-A2 Products

Blogs on Pappalysin-2/PAPP-A2

There are no specific blogs for Pappalysin-2/PAPP-A2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Effects of gene disruption on PAPP-A2 protein.Shown are Western blots of 9 representative (A) placentae and (B) embryos at embryonic day 12.5 from embryos homozygous for wild-type Pappa2 (+/+), heterozygous (+/−) or homozygous for the disruption (−/−). The nitrocellulose membrane was scanned for fluorescence at 700 and 800 nm simultaneously, with fluorescence at 700 nm corresponding to actin (at approximately 40 kDa) and fluorescence at 800 nm corresponding to PAPP-A2 (at approximately 250 kDa). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Pappalysin-2/PAPP-A2 Antibody
AF1668

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Pappalysin-2/PAPP-A2 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol PAPPA2
Uniprot