Recombinant Human Neurolysin Protein, CF

• 6 months from date of receipt, -20 to -70 °C as supplied.
• 3 months, -20 to -70 °C under sterile conditions after opening.

• Assay Buffer: 25 mM Tris, 150 mM NaCl, pH 7.5
• Recombinant Human Neurolysin (rhNeurolysin) (Catalog # 3814-ZN)
• Substrate: MCA-Pro-Leu-Gly-Pro-D-Lys(DNP)-OH (Bachem, Catalog # M-2270), 2 mM stock in DMSO
• F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
• Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

1. Dilute rhNeurolysin to 5 µg/mL in Assay Buffer.
2. Dilute Substrate to 50 µM in Assay Buffer.
3. Load into a black well plate 50 µL of 5 µg/mL rhNeurolysin, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 50 µM Substrate.
4. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
5. Calculate specific activity:

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

• rhNeurolysin: 0.25 µg
• Substrate: 25 µM