![Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.](http://images.novusbio.com/fullsize/Thimet-Oligopeptidase-Antibody-4D6-Western-Blot-NB400-146-img0003.jpg "Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.")
| Reactivity | Hu, Mu, Rt, Pm, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 4D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.54 mg/ml |
| Background | Thimet oligopeptidase, a widely distributed enzyme in most species, plays a role in the metabolism of short neuropeptides (under 20 a.a. residues long). Thimet Oligopeptidase has an influential role in cytoplasmic peptide degradation. Other functions of Thimet Oligopeptidase include the degradation of amyloid precursor protein and the production of amyloidogenic fragments. |
| Immunogen | A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888] |
| Localization | Cytoplasmic |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | THOP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine and 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.54 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Thimet Oligopeptidase/THOP1 Antibody (NB400-146)Find related products by research area.
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