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Recombinant Human MMP-9 Activated Protein, CF

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Recombinant Human MMP-9 Activated (Catalog # 11602-MP) is measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (ES001).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human MMP-9 Activated Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001). The specific activity is >1000 pmol/min/μg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human MMP-9 protein
Ala20-Asp707 (Gln279Arg)
The proform was activated.
Accession #
N-terminal Sequence
Phe107 & Gly547
Structure / Form
Activated
Protein/Peptide Type
Recombinant Enzymes
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
SDS-PAGE
60-85, 38-42, 16-21 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% Brij-35 (w/v), pH 7.5 (TCNB)
  • Recombinant Human MMP-9 Activated (rhMMP-9) (Catalog # 11602-MP)
  • Substrate: MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 (Catalog # ES001)
  • Black 96-well plate
  • Plate Reader with Fluorescence Read Capability
  1. Dilute rhMMP-9 to 0.4 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 µM in Assay Buffer.
  3. Load 50 µL of 0.4 µg/mL rhMMP-9 into a plate and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 20 µM Substrate.
  4. Read at excitation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

   

  *Adjusted for Substrate Blank
  **Derived using calibration standard MCA-Pro-Leu-OH.
Per Well:
  • rhMMP-9: 0.020 μg
  • Substrate: 10 µM


























Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MMP-9 Activated Protein, CF

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

Recombinant human Active Matrix Metalloproteinase 9 (MMP-9), also known as gelatinase B, is a member of the MMP family of zinc and calcium-dependent endopeptidases. MMP-9 protein is synthesized as a pre-proenzyme in specific cells such as neutrophils, macrophages, fibroblasts and endothelial cells (1). Expressed MMP-9 contains a signal peptide to transport it to the extracellular matrix (ECM), a hinge region, a propeptide region, a catalytic domain, and a hemopexin-like domain that is important for substrate recognition (2, 3).  In addition to having three fibronectin type II domains that contribute to substrate binding and an active site, the catalytic domain of MMP-9 also contains a zinc-binding region that interacts with a cysteine in the propeptide to maintain latency. Consequently, removal of the propeptide through cleavage by proteases in the ECM, such as MMP-3, activates the protein (2, 3). MMP-9 has specificity for targets containing an established preferred consensus sequence (3-5) and has a broad range of substrates within the ECM including gelatin, collagen, elastin that contributes to its role in ECM remodeling and extracellular domain cell surface protein release from the plasma membrane (3). Due to its activity in the ECM, MMP-9 plays a role in many biological processes and can serve as a biomarker in tumor invasion and mestastasis (3, 6) of many cancers including colon, ovarian, breast, osteosarcoma, and lung cancers (7-11) making it a therapeutic target (6, 8, 12). MMP-9 modeling of the ECM also leads to a pivotal role in other inflammation- and autoimmune-related diseases (3, 13, 14).
  1. Vandooren, J. et al. (2013) Crit. Rev. Biochem. Mol. Biol.  48: 222.
  2. Roeb, E. et al. (2002) J. Biol. Chem. 277: 50326.
  3. Huang, H. (2018) Sensors. 18:3249.
  4. Kridel, S.J. et al. (2001) J. Biol. Chem. 276: 20572.
  5. Prudova, A. et al. (2010) Mol. Cell Proteom. 9:894.
  6. Kalali, D. (2023) Glob. Med. Genet. 10:48.
  7. Hu, X. et al. (2012) Arch. Glynecol. Obstet. 286:1537.
  8. Wang, J. et al. (2014) Clin. Chim. Acta. 433:225.
  9. Blanco-Prieto, S. et al. (2017) BMC Cancer 17:823.
  10. Liang, S. and L. Chang (2018) Biomark. Med. 12:393.
  11. Malik, S. et al. (2024) Sci. Rep. 14:15117.
  12. Roy, R. et al. (2009) J. Clin. Oncol. 27:5287.
  13. Ram, M. et al. (2006) J. Clin. Immunol. 26:299.
  14. Kim, I.S. et al. (2023) Curr. Med. Chem. 30:2075.

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FAQs for MMP-9 (11602-MP). (Showing 1 - 1 of 1 FAQ).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

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