Recombinant Human IL-12/IL-23 p40 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions (1). It is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Human IL-12p40 is a 40 kDa glycoprotein that shows considerable structural similarity to the extracellular domain of hematopoietin receptors (2). It is synthesized as a 328 amino acid (aa) precursor with a 22 aa signal sequence and a 306 aa mature region that contains a 92 aa fibronectin type III domain and an 84 aa Ig C2-like region and has a high degree of structural homology to type I cytokine receptors. There are two intrachain disulfide bonds and four potential N-linked glycosylation sites (3). Once made, it can exist in multiple forms including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12) (1, 4, 5). Mature human IL-12p40 shows 66% aa sequence identity to mouse and rat IL-12p40 respectively. The secreted form of the p40 subunit inhibits IL-23 functions and abrogates IL-23-mediated anti-tumor effects (6). Characterization of the IL-12p40 proteins for binding and bioactivity showed that both the p40 monomer and dimer inhibited IL-12 binding to IL-12R (7).

Array 11407-IL

• IL-12/IL-23 p40 Antibodies
• IL-12/IL-23 p40 ELISA Kits
• IL-12/IL-23 p40 ELISAs
• IL-12/IL-23 p40 Proteins and Enzymes