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Recombinant Human FGF-4 Protein, CF

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Recombinant Human FGF-4 (Catalog # 235‑F4/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.25‑1.25 ng/mL.
1 μg/lane of Recombinant Human FGF-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human FGF-4 Protein, CF Summary

Additional Information
A New and Improved rh FGF-4 is Now Available! It has enhanced purity!
Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.25-1.25 ng/mL.
Source
E. coli-derived human FGF-4 protein
Ser54-Leu206
Accession #
N-terminal Sequence
Ser54 & Leu55
Protein/Peptide Type
Recombinant Proteins
Gene
FGF4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
16 kDa, reducing conditions
Publications
Read Publications using
235-F4/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-4 Protein, CF

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST

Background

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

  1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  3. Niswander, L. and G.R. Martin (1992) Development 114:755.
  4. Feldman, B. et al. (1995) Science 267:246.
  5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
  6. Sun, X. et al. (2002) Nature 418:501.
  7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
  8. Yu, K and D.M. Ornitz (2008) Development 135:483.
  9. Mariani, F.V. et al. (2008) Nature 453:401.
  10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
  11. Kunath, T. et al. (2007) Development 134:2895.
  12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
  13. Hajitou, A. et al. (1998) Oncogene 17:2059.
  14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

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Publications for FGF-4 (235-F4/CF)(65)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Porcine, Transgenic Mouse, Xenopus.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(57)
Cell Culture
(7)
In Vivo
(1)
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Human
(43)
Mouse
(15)
Rat
(3)
Chicken
(2)
Porcine
(1)
Transgenic Mouse
(1)
Xenopus
(2)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using 235-F4/CF Applications Species
A Rössig, K Hill, W Nörenberg, S Weidenbach, S Zierler, M Schaefer, T Gudermann, V Chubanov Pharmacological agents selectively acting on the channel moieties of TRPM6 and TRPM7 Cell Calcium, 2022-08-17;106(0):102640. 2022-08-17 [PMID: 36030694] (Cell Culture, Human) Cell Culture Human
J Seong, J Frias-Alde, V Holzmann, H Kagawa, G Sestini, H Heidari Kh, Y Scholte Op, M Kip, SJ Pradhan, L Verwegen, J Vivié, L Li, A Alemany, J Korving, F Darmis, A van Oudena, D Ten Berge, N Geijsen, NC Rivron Epiblast inducers capture mouse trophectoderm stem cells in�vitro and pattern blastoids for implantation in utero Cell Stem Cell, 2022-07-07;29(7):1102-1118.e8. 2022-07-07 [PMID: 35803228] (Bioassay, Mouse) Bioassay Mouse
J Jeong, TH Kim, M Kim, YK Jung, KS Kim, S Shim, H Jang, WI Jang, SB Lee, D Choi Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids Biology, 2022-03-23;11(4):. 2022-03-23 [PMID: 35453693] (Bioassay, Human) Bioassay Human
S Xia, Z Bozóky, M Di Paola, O Laselva, S Ahmadi, JX Jiang, AL Pitstick, C Jiang, D Rotin, CN Mayhew, NL Jones, CE Bear High-Throughput Functional Analysis of CFTR and Other Apically Localized Proteins in iPSC-Derived Human Intestinal Organoids Cells, 2021-12-04;10(12):. 2021-12-04 [PMID: 34943927] (Bioassay, Human) Bioassay Human
V Chesnokova, S Zonis, A Apostolou, HQ Estrada, S Knott, K Wawrowsky, K Michelsen, A Ben-Shlomo, R Barrett, V Gorbunova, K Karalis, S Melmed Local non-pituitary growth hormone is induced with aging and facilitates epithelial damage Cell Reports, 2021-12-14;37(11):110068. 2021-12-14 [PMID: 34910915] (Bioassay, Human) Bioassay Human
AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021-12-01;0(0):. 2021-12-01 [PMID: 34856121] (Bioassay, Human) Bioassay Human
MM Ali, SA Khater, AA Fayed, D Sabry, SF Ibrahim Apoptotic endocrinal toxic effects of perchlorate in human placental cells Toxicology reports, 2021-04-17;8(0):863-870. 2021-04-17 [PMID: 33948439] (Cell Culture, Human) Cell Culture Human
K Husain, D Coppola, CS Yang, MP Malafa Farnesyl dimethyl chromanol targets colon cancer stem cells and prevents colorectal cancer metastasis Scientific Reports, 2021-01-26;11(1):2185. 2021-01-26 [PMID: 33500430] (Bioassay, Human) Bioassay Human
SK Sarvestani, S Signs, B Hu, Y Yeu, H Feng, Y Ni, DR Hill, RC Fisher, S Ferrandon, RK DeHaan, J Stiene, M Cruise, TH Hwang, X Shen, JR Spence, EH Huang Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity Nature Communications, 2021-01-11;12(1):262. 2021-01-11 [PMID: 33431859] (Bioassay, Human) Bioassay Human
E Posfai, JP Schell, A Janiszewsk, I Rovic, A Murray, B Bradshaw, T Yamakawa, T Pardon, M El Bakkali, I Talon, N De Geest, P Kumar, SK To, S Petropoulo, A Jurisicova, V Pasque, F Lanner, J Rossant Evaluating totipotency using criteria of increasing stringency Nature Cell Biology, 2021-01-08;23(1):49-60. 2021-01-08 [PMID: 33420491] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Show All 65 Publications.

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Bioinformatics

Gene Symbol FGF4
Uniprot