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Recombinant Equine TNF-alpha Protein

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Product Details

Summary
Reactivity EqSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price
Carrier Free
1814-ET-025/CF
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Recombinant Equine TNF-alpha Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.025-0.1 ng/mL.
Source
E. coli-derived equine TNF-alpha protein
Leu78-Leu234, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1814-ET in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Equine TNF-alpha Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Equine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD) (3). Within the ECD, equine TNF-alpha shares 69% - 88% aa sequence identity with bovine, canine, cotton rat, feline, human, mouse, porcine, rat, and rhesus TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Su, X.Z. et al. (1991) Gene 107:319.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Recombinant Equine
TNF-alpha Protein
1814-ET
Recombinant Equine TNF-alpha Protein
Species: Eq
Applications: Bioactivity

Publications for TNF-alpha (1814-ET)(8)

We have publications tested in 2 confirmed species: Equine, N/A.

We have publications tested in 3 applications: Bioassay, Cell Culture, Reference Standard.


Filter By Application
Bioassay
(5)
Cell Culture
(2)
Reference Standard
(1)
All Applications
Filter By Species
Equine
(7)
N/A
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using 1814-ET Applications Species
NA Moyo, D Westcott, R Simmonds, F Steinbach Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells Viruses, 2023-01-16;15(1):. 2023-01-16 [PMID: 36680295] (Bioassay, Equine) Bioassay Equine
I Cvitas, S Oberhaensl, T Leeb, E Marti Equine keratinocytes in the pathogenesis of insect bite hypersensitivity: Just another brick in the wall? PLoS ONE, 2022-08-01;17(8):e0266263. 2022-08-01 [PMID: 35913947] (Bioassay, Equine) Bioassay Equine
B Jeremiasse, C Matta, CR Fellows, DJ Boocock, JR Smith, S Liddell, F Lafeber, WE van Spil, A Mobasheri Alterations in the chondrocyte surfaceome in response to pro-inflammatory cytokines BMC Mol Cell Biol, 2020-06-26;21(1):47. 2020-06-26 [PMID: 32586320] (Bioassay, Equine) Bioassay Equine
A Lange-Cons, P Romele, M Magatti, A Silini, A Idda, NA Martino, F Cremonesi, O Parolini Priming with inflammatory cytokines is not a prerequisite to increase immune-suppressive effects and responsiveness of equine amniotic mesenchymal stromal cells Stem Cell Res Ther, 2020-03-04;11(1):99. 2020-03-04 [PMID: 32131892] (Cell Culture, Equine) Cell Culture Equine
LM Martin, PJ Johnson, JR Amorim, AE DeClue Effects of Orally Administered Resveratrol on TNF, IL-1beta, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study Int J Mol Sci, 2020-02-20;21(4):. 2020-02-20 [PMID: 32093379] (Reference Standard, N/A) Reference Standard N/A
L Barrachina, A Cequier, A Romero, A Vitoria, P Zaragoza, FJ Vázquez, C Rodellar Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility Stem Cell Res Ther, 2020-02-07;11(1):52. 2020-02-07 [PMID: 32028995] (Cell Culture, Equine) Cell Culture Equine
LJ Berghaus, S Giguère, AI Bordin, ND Cohen Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages Cytokine, 2017-12-12;102(0):7-11. 2017-12-12 [PMID: 29245049] (Bioassay, Equine) Bioassay Equine
Huang H, Lavoie-Lamoureux A, Moran K, Lavoie JP IL-4 stimulates the expression of CXCL-8, E-selectin, VEGF, and inducible nitric oxide synthase mRNA by equine pulmonary artery endothelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-01;292(5):L1147-54. 2007-05-01 [PMID: 17494951] (Bioassay, Equine) Bioassay Equine

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FAQs for TNF-alpha (1814-ET). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Bioinformatics

Gene Symbol TNF
Uniprot