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Recombinant Bovine IFN-gamma Protein, CF

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Bovine IFN-gamma Protein, CF Summary

Details of Functionality
Measured in an anti-viral assay using MDBK bovine kidney epithelial cells infected with vesicular stomatitis virus (VSV). Murkami, K. et al. (2001) Cytokine 13:18. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
E. coli-derived bovine IFN-gamma protein
Gln24-Thr166 & Gln24-Arg162, both with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IFNG
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2300-BG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Bovine IFN-gamma Protein, CF

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma . IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Cerretti, D.P. et al. (1986) J. Immunol. 136:4561.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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2300-BG/CF
Species: Bv
Applications: Bioactivity

Publications for IFN-gamma (2300-BG/CF)(5)

We have publications tested in 3 confirmed species: Human, Bovine, Ovine.

We have publications tested in 2 applications: Bioassay, ELISA Developmet.


Filter By Application
Bioassay
(4)
ELISA Developmet
(1)
All Applications
Filter By Species
Human
(1)
Bovine
(3)
Ovine
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 2300-BG/CF Applications Species
SJ Gilbert, EJ Blain, DJ Mason Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms Biochemistry and Biophysics Reports, 2022-09-07;32(0):101323. 2022-09-07 [PMID: 36105611] (Bioassay, Bovine) Bioassay Bovine
RG Schaut, MV Palmer, PM Boggiatto, IT Kudva, CL Loving, VK Sharma Mucosal IFN&amp;gamma production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle Scientific Reports, 2021-05-07;11(1):9769. 2021-05-07 [PMID: 33963240] (Bioassay, Human) Bioassay Human
W Wheat, L Chow, V Rozo, J Herman, K Still Broo, A Colbath, R Hunter, S Dow Non-specific protection from respiratory tract infections in cattle generated by intranasal administration of an innate immune stimulant PLoS ONE, 2020-06-25;15(6):e0235422. 2020-06-25 [PMID: 32584899] (Bioassay, Bovine) Bioassay Bovine
O Huaman, J Bahamonde, B Cahuascanc, M Jervis, J Palomino, CG Torres, OA Peralta Immunomodulatory and immunogenic properties of mesenchymal stem cells derived from bovine fetal bone marrow and adipose tissue Res. Vet. Sci., 2019-03-21;124(0):212-222. 2019-03-21 [PMID: 30925336] (Bioassay, Bovine) Bioassay Bovine
A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis Stem Cell Res Ther, 2017-02-07;8(1):22. 2017-02-07 [PMID: 28173831] (ELISA Developmet, Ovine) ELISA Developmet Ovine

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FAQs for IFN-gamma (2300-BG/CF). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Additional IFN-gamma Products

Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol IFNG
Uniprot