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Rat Total MMP-9 Quantikine ELISA Kit

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Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
HRP

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Rat Total MMP-9 Quantikine ELISA Kit Summary

Background
The Quantikine Rat Total MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total rat MMP-9 (Pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat MMP-9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obt...ained using natural rat MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat MMP-9.
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Specificity
Natural and recombinant rat MMP-9 (Pro-, active, and TIMP-complexed)
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Mmp9

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using RMP900.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat Total MMP-9 Quantikine ELISA Kit

  • 92 kDa gelatinase
  • 92 kDa type IV collagenase
  • CLG4B
  • EC 3.4.24
  • EC 3.4.24.35
  • Gelatinase B
  • GELB
  • macrophage gelatinase
  • MANDP2
  • matrix metallopeptidase 9
  • matrix metalloproteinase 9
  • matrix metalloproteinase-9
  • MMP9
  • MMP-9
  • type V collagenase

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MMP-9 (RMP900)(15)

We have publications tested in 1 confirmed species: Rat.


Filter By Application
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Filter By Species
Rat
(15)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using RMP900 Applications Species
Krzywonos-Zawadzka, A;Olejnik, A;Sawicki, G;Bil-Lula, I; In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats Biomedicines 2024-03-13 [PMID: 38540247] (Rat) Rat
Tammam, BMH;Habotta, OA;El-Khadragy, M;Abdel Moneim, AE;Abdalla, MS; Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms Heliyon 2023-09-01 [PMID: 37662797] (Rat) Rat
Kim, HR;Lee, SH;Noh, EM;Choi, B;Seo, HY;Jang, H;Kim, SY;Park, MH; Therapeutic Effect of Enzymatically Hydrolyzed Cervi Cornu Collagen NP-2007 and Potential for Application in Osteoarthritis Treatment International journal of molecular sciences 2023-07-19 [PMID: 37511425] (Rat) Rat
A Radajewska, J Szyller, J Niewiadoms, A Noszczyk-N, I Bil-Lula Punica granatum L. Polyphenolic Extract as an Antioxidant to Prevent Kidney Injury in Metabolic Syndrome Rats Oxidative Medicine and Cellular Longevity, 2023-01-05;2023(0):6144967. 2023-01-05 [PMID: 36644578] (Rat) Rat
X Xu, D Wang, Z Han, B Wang, W Gao, Y Fan, F Li, Z Zhou, C Gao, J Xiong, S Zhou, S Zhang, G Yang, R Jiang, J Zhang A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma Brain research bulletin, 2021-04-28;172(0):108-119. 2021-04-28 [PMID: 33932488] (Rat) Rat
A ?ukomska, I Baranowska, K Dec, A Pilutin, M Tarnowski, K Jakubczyk, W ?wiere??o, M Skórka-Maj, D Chlubek, I Gutowska Changes in Gene and Protein Expression of Metalloproteinase-2 and -9 and Their Inhibitors TIMP2 and TIMP3 in Different Parts of Fluoride-Exposed Rat Brain International Journal of Molecular Sciences, 2020-12-31;22(1):. 2020-12-31 [PMID: 33396569] (Rat) Rat
Y Yuan, H Naito, K Kitamori, S Hashimoto, T Asano, T Nakajima The antihypertensive agent hydralazine reduced extracellular matrix synthesis and liver fibrosis in nonalcoholic steatohepatitis exacerbated by hypertension PLoS ONE, 2020-12-14;15(12):e0243846. 2020-12-14 [PMID: 33315911] (Rat) Rat
AE Bulboac?, AS Porfire, LR Tefas, PM Boarescu, SD Bolboac?, IC St?nescu, AC Bulboac?, G Dogaru Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus Molecules, 2019-02-27;24(5):. 2019-02-27 [PMID: 30818888] (Rat) Rat
A Krzywonos-, A Franczak, A Olejnik, M Radomski, JF Gilmer, G Sawicki, M Wo?niak, I Bil-Lula Cardioprotective effect of MMP-2-inhibitor-NO-donor hybrid against ischaemia/reperfusion injury J. Cell. Mol. Med., 2019-02-07;0(0):. 2019-02-07 [PMID: 30729745] (Rat) Rat
RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy J. Neurosci., 2018-04-09;0(0):. 2018-04-09 [PMID: 29632167] (Rat) Rat
Show All 15 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 (RMP900). (Showing 1 - 1 of 1 FAQs).

  1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
    • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

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Blogs on MMP-9.

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Bioinformatics

Gene Symbol Mmp9