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Rat IFN-gamma Quantikine ELISA Kit

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Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
HRP

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Rat IFN-gamma Quantikine ELISA Kit Summary

Background
The Quantikine Rat IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IFN-gamma levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant rat IFN-gamma accurately. Results obtained using natural rat IFN-g...amma showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IFN-gamma.
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Specificity
Natural and recombinant rat IFN-gamma
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Ifng

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using RIF00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IFN-gamma Quantikine ELISA Kit

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-gamma (RIF00)(32)

We have publications tested in 1 confirmed species: Rat.


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Rat
(32)
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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using RIF00 Applications Species
Kirkland, JM;Patel, I;Ardeshna, MS;Kopec, AM; Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205376] (Rat) Rat
YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration Bioactive materials, 2021-07-27;9(0):475-490. 2021-07-27 [PMID: 34820584] (Rat) Rat
L Zheng, R Tang, L Shi, M Zhong, Z Zhou Vagus nerve stimulation ameliorates L-NAME-induced preeclampsia-like symptoms in rats through inhibition of the inflammatory response BMC pregnancy and childbirth, 2021-03-04;21(1):177. 2021-03-04 [PMID: 33663436] (Rat) Rat
F Yaghouby, K Jang, U Hoang, S Asgari, S Vasudevan Sex Differences in Vagus Nerve Stimulation Effects on Rat Cardiovascular and Immune Systems Front Neurosci, 2020-11-06;14(0):560668. 2020-11-06 [PMID: 33240036] (Rat) Rat
B Xiao, Y Zhang, S Liu, J Ding, Z Yu, T Wang, B Song Nuclear Receptor Subfamily 4 Group A Member 1 Overexpression Prolongs Free Flap Allotransplant Graft Survival by Inducing T-Cell Anergy in the Rat Transplant. Proc., 2020-09-09;0(0):. 2020-09-09 [PMID: 32919804] (Rat) Rat
S Sekar, SK Panchal, NK Ghattamane, L Brown, R Crawford, Y Xiao, I Prasadam Dietary Saturated Fatty Acids Modulate Pain Behaviour in Trauma-Induced Osteoarthritis in Rats Nutrients, 2020-02-18;12(2):. 2020-02-18 [PMID: 32085385] (Rat) Rat
E Ramírez, C Sánchez-Ma, MA Mayoral, L Mendieta, V Alatriste, A Patricio-M, ID Limón Neuroinflammation induced by the peptide amyloid-? (25-35) increase the presence of galectin-3 in astrocytes and microglia and impairs spatial memory Neuropeptides, 2019-02-14;0(0):. 2019-02-14 [PMID: 30795916] (Rat) Rat
BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus) PLoS ONE, 2017-12-13;12(12):e0188779. 2017-12-13 [PMID: 29236720] (Rat) Rat
JW Jeong, HH Lee, J Kim, EO Choi, H Hwang-Bo, HJ Kim, MY Kim, KI Ahn, GY Kim, KW Lee, KY Kim, SG Kim, SH Hong, C Park, HJ Cha, YH Choi Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate?induced osteoarthritis rats Mol Med Rep, 2017-07-21;0(0):. 2017-07-21 [PMID: 29067461] (Rat) Rat
WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro Mol Med Rep, 2017-03-15;15(5):2551-2559. 2017-03-15 [PMID: 28447750] (Rat) Rat
Show All 32 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IFN-gamma (RIF00). (Showing 1 - 1 of 1 FAQs).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Additional IFN-gamma Products

Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Bioinformatics

Gene Symbol Ifng