![Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12V-Mutant-Western-Blot-NBP3-13668-img0007.jpg "Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12V Mutant) antibody [HL169] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL169 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding mutant G12V of human K-Ras. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - BxPC-3 xenograft (left) and CFPAC-1 xenograft (right). RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12V-Mutant-Immunohistochemistry-Paraffin-NBP3-13668-img0004.jpg "Immunohistochemistry-Paraffin: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - BxPC-3 xenograft (left) and CFPAC-1 xenograft (right). RAS (G12V Mutant) stained by RAS (G12V Mutant) antibody [HL169] diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Ras Antibody (HL169) - G12D Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (NBP3-13668) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ras-Antibody-HL169-G12D-Mutant-Western-Blot-NBP3-13668-img0003.jpg "Western Blot: Ras Antibody (HL169) - G12V Mutant [NBP3-13668] - Ras Antibody (HL169) - G12D Mutant [NBP3-13668] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12V Mutant) antibody [HL1] (NBP3-13668) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
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