![Western Blot: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAMP2.](http://images.novusbio.com/fullsize/RAMP2-Antibody-OTI4E5-Azide-and-BSA-Free-Western-Blot-NBP2-73796-img0001.jpg "Western Blot: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAMP2.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | OTI4E5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinat protein of human RAMP2(NP_005845) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAMP2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
![Immunohistochemistry EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/EN-RAGE_AF1052_Immunohistochemistry_6671.jpg)
![Western Blot EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/ENRAGE_AF1052_Western_Blot_19098.jpg)
![Simple Western EN-RAGE/S100A12 Antibody [Unconjugated]](https://images.novusbio.com/images2/af1052_human-en-rage-affinity-purified-polyclonal-ab-simple-western-2672021152820.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RAMP2 |
![Immunohistochemistry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in](http://images.novusbio.com/fullsize/RAMP2-Antibody-OTI4E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73796-img0003.jpg "Immunohistochemistry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in")
![Flow Cytometry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RAMP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/RAMP2-Antibody-OTI4E5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73796-img0002.jpg "Flow Cytometry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RAMP2 antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - Staining of paraffin-embedded Human Kidney tissue using anti-RAMP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RAMP2-Antibody-OTI4E5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73796-img0004.jpg "Immunohistochemistry: RAMP2 Antibody (OTI4E5) - Azide and BSA Free [NBP2-73796] - Staining of paraffin-embedded Human Kidney tissue using anti-RAMP2 mouse monoclonal antibody.")
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![Western Blot RAMP1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RAMP1_AF6428_Western_Blot_9866.jpg)
![Immunohistochemistry RAMP1 Antibody [Unconjugated]](https://images.novusbio.com/images2/af6428_human-ramp1-affinity-purified-polyclonal-ab-immunohistochemistry-27102020154839.jpg)
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![Immunocytochemistry/ Immunofluorescence Calcitonin R Antibody (462802) [Unconjugated]](https://images.novusbio.com/images/mab4614_human-calcitonin-r-mab-clone-462802-41202410484315.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")