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RAMP2 Antibody (OTI4E5)

Images

 
Western Blot: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - FFPE gingival tissue from Macaca mulatta was stained with RAMP2 Antibody (4E5) as described in "Other experimental details". Clear membranous and ...read more
Flow Cytometry: RAMP2 Antibody (4E5) [NBP2-01853] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAMP2 antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: RAMP2 Antibody (4E5) [NBP2-01853] - Staining of paraffin-embedded Human Kidney tissue using anti-RAMP2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RAMP2 Antibody (OTI4E5) Summary

Immunogen
Full length human recombinat protein of human RAMP2(NP_005845) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:1000
Theoretical MW
15.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-01853 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAMP2 Antibody (OTI4E5)

  • calcitonin receptor-like receptor activity modifying protein 2
  • Calcitonin-receptor-like receptor activity-modifying protein 2
  • CRLR activity-modifying protein 2
  • RAMP2
  • receptor (calcitonin) activity modifying protein 2
  • receptor (G protein-coupled) activity modifying protein 2
  • receptor activity modifying protein 2
  • receptor activity-modifying protein 2
  • receptor-activity-modifying protein 2

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAMP2 Antibody (NBP2-01853) (0)

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Review for RAMP2 Antibody (NBP2-01853) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Macaca mulatta.

Reviews using NBP2-01853:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Macaca mulatta
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin RAMP2 NBP2-01853
Enlarge
5
reviewed by:
Verified Customer
IHC-P Macaca mulatta 02/20/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedGingival tissue
SpeciesMacaca mulatta
LotA002

Comments

CommentsHIER: Diva Decloaker 5 minutes + 45 minutes cool down
Antibody dilution: 1:150
Antibody incubation: Overnight @ 4 deg C
DAB: 5 min

Please use this review for an Innovators Reward Program.
Also, please use code MBCS17 (to get an additional 100 points per review). Thank you!

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RAMP2 Antibody (NBP2-01853) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
4
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2
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1
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Verified Customer
02/20/2017
Application: IHC-P
Species: Macaca mulatta

Bioinformatics

Gene Symbol RAMP2
Entrez