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RAG1 Antibody (1D9) - BSA Free

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Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against HEK293 (1) and RAG1 (AA: 818-868)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: RAG1 Antibody (1D9) [NBP2-37449] - Western blot analysis using RAG1 mAb against human RAG1 recombinant protein. (Expected MW is 31.6 kDa)
ELISA: RAG1 Antibody (1D9) [NBP2-37449] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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RAG1 Antibody (1D9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human RAG1 (AA: 818-868) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAG1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
Theoretical MW
119 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RAG1 Antibody (1D9) - BSA Free

  • MGC43321
  • RAG-1
  • recombination activating gene 1
  • RING finger protein 74
  • RNF74recombination activating protein 1
  • V(D)J recombination-activating protein 1

Background

The protein encoded by the RAG1 gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAG1