![Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23359_rabbit-raf-1-p-ser471-pab-19920231915416.jpg "Western Blot: Raf-1 [p Ser471] Antibody [NBP3-23359] - Western blot of human Jurkat cells treated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated (lanes 2 & 4) with lambda phosphatase and probed with anti-Raf-1 (N-terminal region) (lanes 1 & 2) or anti-Raf-1 (Ser-471) (lanes 3 & 4).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human Raf-1. This sequence has high homology with similar regions in rat and mouse Raf-1, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432). [UniProt# P04049] |
| Modification | p Ser471 |
| Specificity | This antibody was cross-adsorbed to phospho-Raf-1 (Ser-338) peptide before affinity purification using phospho-Raf-1 (Ser-471) peptide. The purified antibody detects a band at 74 kDa corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAF1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 74 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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![Raf-1 [p Ser471] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Raf-1 [p Ser471] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for Raf-1 Antibody (NBP3-23359)Find related products by research area.
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| Gene Symbol | RAF1 |
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