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Raf-1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human testis shows weak positivity in nuclear speckles in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cerebellum shows moderate positivity in nuclear speckles in Purkinje cells.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cervix shows moderate positivity in nuclear speckles in squamous epithelial cells.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human rectum shows moderate positivity in nuclear speckles in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Raf-1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQ
Predicted Species
Mouse (96%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Raf-1 Protein (NBP1-85462PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Raf-1 Antibody

  • cRaf
  • c-Raf
  • c-Raf-1
  • CRAFraf proto-oncogene serine/threonine protein kinase
  • EC 2.7.11
  • EC 2.7.11.1
  • NS5
  • Proto-oncogene c-RAF
  • RAF proto-oncogene serine/threonine-protein kinase
  • RAF
  • Raf1
  • Raf-1
  • Raf-1Oncogene RAF1
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • v-Raf-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RAF1