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Rad21 Antibody (CM110-2C10) [PE]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
CM110-2C10
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

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Rad21 Antibody (CM110-2C10) [PE] Summary

Immunogen
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
Specificity
NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD21
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Rad21 Antibody (CM110-2C10) [PE]

  • double-strand-break repair protein rad21 homolog
  • hHR21FLJ25655
  • HR21
  • KIAA0078RAD21 (S. pombe) homolog
  • MCD1
  • Nuclear matrix protein 1
  • NXP-1
  • NXP1HRAD21
  • protein involved in DNA double-strand break repair
  • RAD21 homolog (S. pombe)
  • SCC1 homolog
  • SCC1FLJ40596

Background

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD21