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Rad21 Antibody (CM110-2C10) [DyLight 650]

 
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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western
Clone
CM110-2C10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-386AF350
Alexa Fluor 405
NB100-386AF405
Alexa Fluor 488
NB100-386AF488
Alexa Fluor 532
NB100-386AF532
Alexa Fluor 594
NB100-386AF594
Alexa Fluor 647
NB100-386AF647
Alexa Fluor 700
NB100-386AF700
Alexa Fluor 750
NB100-386AF750
Allophycocyanin
NB100-386APC
Biotin
NB100-386B
CoraFluor 1
NB100-386CL1
DyLight 350
NB100-386UV
DyLight 405
NB100-386V
DyLight 488
NB100-386G
DyLight 550
NB100-386R
DyLight 594
NB100-386DL594
DyLight 680
NB100-386FR
DyLight 755
NB100-386IR
HRP
NB100-386H
Janelia Fluor 525
NB100-386JF525
Janelia Fluor 549
NB100-386JF549
Janelia Fluor 585
NB100-386JF585
Janelia Fluor 635
NB100-386JF635
Janelia Fluor 646
NB100-386JF646
Janelia Fluor 669
NB100-386JF669
PE
NB100-386PE
PerCP
NB100-386PCP
Unconjugated
NB100-386
mFluor Violet 450 SE
NB100-386MFV450
mFluor Violet 500 SE
NB100-386MFV500
mFluor Violet 610 SE
NB100-386MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-386
BSA Free
NB100-386
BSA Free
NB100-386SS
Azide and BSA Free
NBP2-80922
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Rad21 Antibody (CM110-2C10) [DyLight 650] Summary

Immunogen
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
Localization
Nuclear and cytoplasm
Specificity
NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RAD21
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Rad21 Antibody (CM110-2C10) [DyLight 650]

  • double-strand-break repair protein rad21 homolog
  • hHR21FLJ25655
  • HR21
  • KIAA0078RAD21 (S. pombe) homolog
  • MCD1
  • Nuclear matrix protein 1
  • NXP-1
  • NXP1HRAD21
  • protein involved in DNA double-strand break repair
  • RAD21 homolog (S. pombe)
  • SCC1 homolog
  • SCC1FLJ40596

Background

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Rad21 Antibody
(CM110-2C10) [DyLight ...
NB100-386C
Rad21 Antibody (CM110-2C10) [DyLight 650]
Species: Hu, Mu
Applications: WB, Simple Western

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Bioinformatics

Gene Symbol RAD21