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Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

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Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pm, ZeSpecies Glossary
Applications WB, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400). [Swiss-Prot Q15118]
Localization
Mitochondrion
Predicted Species
Rat (100%), Porcine (91%), Bovine (100%), Zebrafish (91%), Chicken (92%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting reported in scientific literature (PMID 28366875)
  • Western Blot 0.5-2 ug/ml
Application Notes
This PDK1 antibody is useful for Western blot, where a band is seen at ~49 kDa representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pyruvate Dehydrogenase Kinase 1/PDK1 Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
Pyruvate Dehydrogenase Kinase 1/PDK1 Protein (NB100-2384PEP)
Publications
Read Publications using
NB100-2384 in the following applications:

  • IB
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25925251).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.2
  • mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
  • PDH kinase 1
  • PDK1
  • PDK-1
  • PKB kinase
  • Pyruvate dehydrogenase kinase isoform 1
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2384)(4)

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WB Video Protocol

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Bioinformatics

Gene Symbol PDK1