![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0008.jpg "Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 expression in transfected 293T cell line by PCK1 monoclonal antibody (M01), clone 3E4.Lane 1: PCK1 transfected lysate(69.2 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 3E4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | PCK1 (AAH23978, 1 a.a. ~ 622 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPPQLQNGLNLSAKVVQGSLDSLPQAVREFLENNAELCQPDHIHICDGSEEENGRLLGQMEEEGILRRLKKYDNCWLALTDPRDVARIESKTVIVTQEQRDTVPIPKTGLSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLSKIGIELTDSPYVVASMRIMTRMGTPVLEALGDGEFVKCLHSVGCPLPLQKPLVNNWPCNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRMASRLAKEEGWLAEHMLVLGITNPEGEKKYLAAAFPSACGKTNLAMMNPSLPGWKVECVGDDIAWMKFDAQGHLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLASGVTITSWKNKEWSSEDGEPCAHPNSRFCTPASQCPIIDAAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPAAKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM |
| Localization | Cytoplasmic |
| Specificity | PCK1 - phosphoenolpyruvate carboxykinase 1 (soluble) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PCK1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PCK1 Antibody (H00005105-M01)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00005105-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of monoclonal antibody to PCK1 on HepG2 cell. Antibody concentration 10 ug/ml.")
![Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Western-Blot-H00005105-M01-img0007.jpg "Western Blot: PCK1 Antibody (3E4) [H00005105-M01] - PCK1 monoclonal antibody (M01), clone 3E4 Analysis of PCK1 expression in HepG2.")
![Immunoprecipitation: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCK1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PCK1-Antibody-3E4-Immunoprecipitation-H00005105-M01-img0006.jpg "Immunoprecipitation: PCK1 Antibody (3E4) [H00005105-M01] - Analysis of PCK1 transfected lysate using anti-PCK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCK1 MaxPab rabbit polyclonal antibody.")
![Western Blot Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Western_Blot_5296.jpg)
![Simple Western Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Simple_Western_16762.jpg)
![Immunocytochemistry Lyp/PTPN22 Antibody [Unconjugated]](https://images.novusbio.com/images2/Lyp_AF3428_Immunocytochemistry__Immunofluorescence_17421.jpg)
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")