![Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB100-56623-img0002.jpg "Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | A synthetic peptide (AAPPLTLEGPVQSHHGTP) corresponding to amino acids 36-53 of human PUMA d (GenBank no. gb|AAK39544.1|AF354656_1 ) and amino acids 196-213 of human PUMA g (GenBank no ref|NP_001120712.1|) was used as the immunogen. |
| Specificity | The amino acid sequence used as immunogen has 100% identity in PUMAd and PUMAg, and 59% identity in PUMAa. For additional information on PUMA isoforms and their sequences, see Nakano et al. 2001. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | BBC3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS containing 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Protein G purified |
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| Publication using NB100-56623 | Applications | Species |
|---|---|---|
| Liang R, Lin M, Menon V et al. Elevated CDKN1a (p21) mediates ?-thalassemia erythroid apoptosis but its loss does not improve ?-thalassemic erythropoiesis Blood advances 2023-09-06 [PMID: 37672319] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for PUMA Antibody (NB100-56623)Find related products by research area.
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A link between Autophagy and Apoptosis: Chat with First Author Brent E. Fitzwalter By Christina Towers, PhD. Autophagy is a cellular recycling process and most often a pro-survival mechanism that regulates cellular homeostasis. On the contrary, apoptosis is an extensively conserved and elaborate pr... Read full blog post. |
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Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis? Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death. Novus Biologicals offers a variet... Read full blog post. |
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Altered expression of BCL2 in cancer Similar to other cell processes, the balance between cell survival and cell death is an important equilibrium that when altered expression of genes can lead to a variety of disease. For example, too little cell death can promote cell overgrowth a... Read full blog post. |
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IRE1 alpha dependent apoptotic-signaling pathway Despite in depth characterization of the role of IRE1 alpha (inositol-requiring enzyme 1 alpha) in activating the unfolded protein response (UPR) in the ER - little is known about the molecular mechanisms by which this ER protein has shown to reg... Read full blog post. |
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Understanding Noxa Regulation of Apoptosis Noxa is a pro-apoptotic gene belonging to the Bcl2 protein family that is unique in that it contains only BH3 domain. The BH3-only subclass of proteins, including proteins like PUMA and Bim in addition to Noxa, regulate the remaining Bcl-2 family memb... Read full blog post. |
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| Gene Symbol | BBC3 |
| Uniprot |
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