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![Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Analysis of PUMA in K562 cells with PUMA antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76639-img0007.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Human breast carcinoma with PUMA antibody at 10 ug/mL. Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunohistochemistry-Paraffin-NBP1-76639-img0009.jpg)
![Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Independent Antibody Validation (IAV) via Protein Expression ProfileLoading: 20 ug of lysates per lane.Antibodies: NBP1-76654 (3 ug/mL), NBP1-76639 (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-76639-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - K562 cells.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76639-img0004.jpg)
![Immunohistochemistry: PUMA Antibody - BSA Free [NBP1-76639] - PUMA Antibody [NBP1-76639] - Analysis of PUMA in human breast tissue with PUMA antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunohistochemistry-NBP1-76639-img0006.jpg)
![Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB100-56370-img0003.jpg "Western Blot: PUMA Antibody [NB100-56370] - Analysis for Puma a (CT) using NB100-56370 at 1:500 against 30 ug/lane of A) Jurkat and B) NIH 3T3 whole cell lysate.")
![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody [NB100-56370] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of a normal mouse lung using PUMA antibody at 1-200 dilution. The signal was developed using HRP-DAB detection and the sections were further counterstained using hematoxylin. The antibody generated a specific diffused cytoplasmic staining in the bronchiolar and alveolar epithelial cells. The representative section is showing alveolar area only.](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunohistochemistry-Paraffin-NB100-56370-img0004.jpg)
![Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Western-Blot-NBP2-67326-img0008.jpg "Western Blot: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of PUMA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: K562 cell lysate")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0003.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BS](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0009.jpg)
![Flow Cytometry: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of Hela cells with PUMA antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Flow-Cytometry-NBP2-67326-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR42-09) [NBP2-67326] - Staining PUMA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunocytochemistry-Immunofluorescence-NBP2-67326-img0002.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded human gastric carcimnoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0006.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (SR42-09) [NBP2-67326] - Analysis of paraffin-embedded mouse stomach tissue using anti-PUMA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PUMA-Antibody-SR42-09-Immunohistochemistry-Paraffin-NBP2-67326-img0007.jpg)
![Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB500-261-img0003.jpg "Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody [NB500-261] - The PUMA antibody was tested at a 1:20 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-261-img0004.jpg)
![Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Western-Blot-NBP3-16268-img0006.jpg "Western Blot: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of extracts of various cell lines, using PUMA Rabbit mAb (NBP3-16268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Analysis of U-2 OS cells using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunocytochemistry-Immunofluorescence-NBP3-16268-img0005.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Mouse spinal cord using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Immunofluorescence analysis of C6 cells using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunocytochemistry-Immunofluorescence-NBP3-16268-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (9C5W5) [NBP3-16268] - Immunofluorescence analysis of NIH-3T3 cells using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunocytochemistry-Immunofluorescence-NBP3-16268-img0004.jpg)
![Immunohistochemistry-Paraffin: PUMA Antibody (9C5W5) [NBP3-16268] - Rat brain using PUMA Rabbit mAb (NBP3-16268) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PUMA-Antibody-9C5W5-Immunohistochemistry-Paraffin-NBP3-16268-img0001.jpg)
![Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB100-56623-img0002.jpg "Western Blot: PUMA Antibody [NB100-56623] - Analysis of PUMA in A) HeLa and B) NIH 3T3 cell lysate using this antibody at 5 ug/ml.")
![Western Blot: PUMA Antibody (SR1298) [NBP3-21667] - Western blot analysis using NBP3-21667 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21667_rabbit-puma-mab-sr1298-57202316112149.jpg "Western Blot: PUMA Antibody (SR1298) [NBP3-21667] - Western blot analysis using NBP3-21667 in K562 cell lysate.")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody (SR1298) [NBP3-21667] - Immunofluorescent analysis of Hela cells, using NBP3-21667.](http://images.novusbio.com/fullsize/nbp3-21667_rabbit-puma-mab-sr1298-6720239533736.jpg)
![Immunohistochemistry: PUMA Antibody (SR1298) [NBP3-21667] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21667.](http://images.novusbio.com/fullsize/nbp3-21667_rabbit-puma-mab-sr1298-5720231554519.jpg)
![Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NBP1-52093-img0002.jpg "Western Blot: PUMA Antibody [NBP1-52093] - (0.1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PUMA Antibody [NB500-230] - WB detection of PUMA in transfected U20S cells using NB500-230 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB500-230-img0002.jpg "Western Blot: PUMA Antibody [NB500-230] - WB detection of PUMA in transfected U20S cells using NB500-230 at a dilution of 1:250. Please note that this antibody is expected to detect alpha as well as the beta forms of this target.")
![Western Blot: PUMA Overexpression Lysate (Adult Normal) [NBL1-07925] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PUMA.](http://images.novusbio.com/fullsize/PUMA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07925-img0002.jpg "Western Blot: PUMA Overexpression Lysate (Adult Normal) [NBL1-07925] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PUMA.")
![Western Blot: PUMA Overexpression Lysate (Adult Normal) [NBP2-11377] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PUMA.](http://images.novusbio.com/fullsize/PUMA-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11377-img0001.jpg "Western Blot: PUMA Overexpression Lysate (Adult Normal) [NBP2-11377] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PUMA.")