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PUM2 Antibody (740610)

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Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PUM2 Monoclonal Antibody (Catalog # MAB7415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PUM2 Monoclonal Antibody (Catalog # MAB7415) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
740610
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7415-SP
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PUM2 Antibody (740610) Summary

Immunogen
E. coli-derived recombinant human PUM2
Pro103-Gly250
Accession # Q8TB72
Specificity
Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PUM1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PUM2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PUM2 Antibody (740610)

  • FLJ36528
  • KIAA0235PUMH2PUML2
  • MGC138251
  • MGC138253
  • PUM2
  • PUMH2
  • pumilio (Drosphila) homolog 2
  • pumilio homolog 2 (Drosophila)
  • pumilio homolog 2
  • Pumilio-2
  • PUML2

Background

The Pumilio proteins PUM1 and PUM2 are RNA binding proteins that are repressors of mRNA translation. PUM2 is present in embryonic stem cells and adult germ cells. Both positive and negative influences of PUM2 on proliferation have been reported. Human PUM2 is a 1066 amino acid (aa), 114 kDa (predicted) protein with 6 potential Ser/Thr phosphorylation sites (aa 82, 136, 178, 182, 184, 587, and 672), an acetylation site (aa 672), and a pumilio domain (aa 706-1048). Within aa 103-250, human PUM2 shares 95% aa sequence identity with mouse and rat PUM2. A potential 987 aa isoform lacks aa 574-652.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PUM2 Antibody
(740610)
MAB7415
PUM2 Antibody (740610)
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol PUM2
Uniprot