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PTS Antibody

Images

 
Genetic Strategies: Western Blot: PTS Antibody [NBP1-32975] - Wild-type (WT) and PTS knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody diluted ...read more
Immunocytochemistry/ Immunofluorescence: PTS Antibody [NBP1-32975] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: PTS Antibody [NBP1-32975] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: PTS Antibody [NBP1-32975] - Sample (30 ug of whole cell lysate) A: H1299 15% SDS PAGE diluted at 1:1000
Western Blot: PTS Antibody [NBP1-32975] - Sample (50 ug of whole cell lysate) A: mouse stomach 15% SDS PAGE; antibody diluted at 1:1000.
Western Blot: PTS Antibody [NBP1-32975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PTS antibody diluted at 1:5000. ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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PTS Antibody Summary

Immunogen
Full length human PTS Recombinant protein.
Predicted Species
Rhesus Macaque (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTS
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:1000-1:10000
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (87%), Bovine (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PTS Antibody

  • 6-pyruvoyl tetrahydrobiopterin synthase
  • 6-pyruvoyltetrahydropterin synthase
  • EC 4.2.3.12
  • FLJ97081
  • PTPSPTP synthase

Background

The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PTS Antibody (NBP1-32975) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Calculators

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Bioinformatics

Gene Symbol PTS