![Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80920-img0001.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC, CyTOF-ready, IHC |
| Clone | 5G10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | The highly conserved Homeo and Prospero domain of human Prox1. [UniProt# Q92786] |
| Epitope | The homeo prospero domain of Prox1 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PROX1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Prox1 Antibody (NBP2-80920)Find related products by research area.
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Read full blog post. |
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Podoplanin (OST8, Glycoprotein (Gp) 36 or 38, Lung Type I Cell Membrane Associated Glycoprotein) Podoplanin is a mucin-type 1 transmembrane glycoprotein found in a wide range of tissues. It appears to be differentially expressed in endothelial cells of lymphatic but not blood vessel origin. In normal skin and kidney, podoplanin co-localizes with ... Read full blog post. |
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| Gene Symbol | PROX1 |
![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic stain](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0004.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic stain")
![Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80920-img0005.jpg "Flow Cytometry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.")
![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0002.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College. Image from the standard format of this antibody.")
![Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80920-img0003.jpg "Immunohistochemistry: Prox1 Antibody (5G10) - Azide and BSA Free [NBP2-80920] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in")
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![N/A VEGF-D [HRP]](https://images.novusbio.com/images2/DATA_VEGFD_DVED00_ELISA_875.jpg)
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")