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Prox1 Antibodies

We offer PROX1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PROX1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PROX1 Antibodies can be used in a variety of model species: Chicken, Human, Mouse, Rat. Use the list below to choose the PROX1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PROX1 polyclonal antibodies and browse our PROX1 monoclonal antibody catalog.

Alternate Names for Prox1 Antibodies

anti-Prox1 antibody, anti-Homeobox prospero-like protein PROX1 antibody, anti-prospero homeobox 1 antibody, anti-prospero homeobox protein 1 antibody, anti-prospero-related homeobox 1 antibody, anti-PROX-1 antibody

10 Results for "prox1" in Primary Antibodies

AF2727

Prox1 Antibody [Unconjugated]

(4 Reviews)

(148 Publications)

100 μg

AF2727

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Prox1 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Prox1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (left panel, red; Catalog # NL001) and counterstained with DAPI (right panel, blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Prox1 at approximately 114 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Prox1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2727) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

RNA-seq identifies the targets of GATA2 in primary human LECs. (A) Principal component analysis (PCA) was performed on RNA-seq data from control shRNA- and shGATA2-infected primary HLECs. A high level of similarity was observed within the groups as indicated by their proximity to each other. (B) Hierarchical clustering shows that approximately 1000 genes were consistently downregulated and 600 genes were upregulated in shGATA2-treated HLECs. (C) GO revealed a list of genes that are likely relevant to the phenotypes observed in mice lacking GATA2. (D) GATA2 was knocked out from a second HLEC line using CRISPR/Cas9. Western blot revealed the lack of GATA2 in the knockout cells (HLEC delta GATA2). In contrast, no obvious differences were observed in the expression of PROX1. Additionally, qRT-PCR revealed the downregulation of miR-126. (A) n=3 independent experiments per shRNA; (D) n=3 independent experiments (antibiotic selection, western blot and qRT-PCR). **P<0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31582413), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates myoblast differentiation.(a) Analysis of Prox1 mRNA and protein during C2C12 myoblast differentiation (indicated by the increase in MyoD1 mRNA). (b) Prox1 protein and mRNA in human myoblasts before and after differentiation. Primary myoblast lines from two different individuals were analysed. (c–f) Analysis of myoblast proliferation and differentiation-related CyclinD1, Myf5 and MyoD mRNAs in shProx1 and shScr transduced primary human myoblasts before (c) and after differentiation for 7d (d), as well as in mouse C2C12 myoblasts before (e) and after (f) differentiation. (g) Myosin staining of C2C12 cells after 7d of differentiation. Note that Prox1 silencing completely blocked myotube development, as only occasional cells expressed myosin and these cells were negative for shProx1-GFP. Data is presented as mean±s.e.m., n=3+3 biological replicates in a–g (all experiments were repeated three times). One-way analysis of variance with repeated measures followed by Tukey's posthoc test and Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Prox1 regulates the slow muscle fibre gene program in skeletal muscle.(a) MyHC I (Myh7) expression in muscle fibres (arrows) in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. (b) Expression of myosin heavy chain and calcium-signalling genes in AAV8-Prox1 and AAV8-Ctrl transduced TA muscle. Note that the expression of slow fibre -specific genes are upregulated and fast MyHC genes downregulated in Prox1 overexpressing muscles. (c) Heat map from GSEA analysis showing that Prox1 overexpression in TA muscle leads to expression of many soleus specific myofibrillar genes (red colour indicates positive and blue negative enrichment). (d) Effect of Prox1 deletion on soleus muscle gene expression (HSA-CreERT2;Prox1fl/fl mice). Note the increased expression of fast MyHCs Myh2 and Myh4 RNAs, whereas slow troponin isoform RNAs and Myh7 RNA are decreased. (e) Deletion of Prox1 in slow soleus muscle with AAV-Cre leads to marked downregulation of MyHC I protein. Data is presented as mean±s.e.m., n=5+5 in a,b, n=3+3 in c, n=4+4 in d,e. Student's two-tailed unpaired t-test, *P<0.05, **P<0.01, ***P<0.001. Both overexpression and deletion experiments were conducted two times on independent mouse cohorts. WT, wild-type. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13124), licensed under a CC-BY license. Not internally tested by R&D Systems.

In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36high LYVE-1+ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36high LYVE-1+ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO+ CLEC4M+ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32426372), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2727

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2727-SP

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NBP1-30045

Prox1 Antibody (5G10) - BSA Free

(5 Publications)

0.1 ml

NBP1-30045

Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry: Prox1 Antibody (5G10) [NBP1-30045] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.

Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.

Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.

Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.

Reactivity:

Hu, Mu, Rt, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ch

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-30045AF350
Alexa Fluor 405	NBP1-30045AF405
Alexa Fluor 488	NBP1-30045AF488
Alexa Fluor 532	NBP1-30045AF532
Alexa Fluor 594	NBP1-30045AF594
Alexa Fluor 647	NBP1-30045AF647
Alexa Fluor 700	NBP1-30045AF700
Alexa Fluor 750	NBP1-30045AF750
Allophycocyanin	NBP1-30045APC
Biotin	NBP1-30045B
CoraFluor 1	NBP1-30045CL1
DyLight 350	NBP1-30045UV
DyLight 405	NBP1-30045V
DyLight 488	NBP1-30045G
DyLight 550	NBP1-30045R
DyLight 594	NBP1-30045DL594
DyLight 650	NBP1-30045C
DyLight 680	NBP1-30045FR
DyLight 755	NBP1-30045IR
FITC	NBP1-30045F
HRP	NBP1-30045H
Janelia Fluor 525	NBP1-30045JF525
Janelia Fluor 549	NBP1-30045JF549
Janelia Fluor 585	NBP1-30045JF585
Janelia Fluor 635	NBP1-30045JF635
Janelia Fluor 646	NBP1-30045JF646
Janelia Fluor 669	NBP1-30045JF669
PE	NBP1-30045PE
mFluor Violet 450 SE	NBP1-30045MFV450
mFluor Violet 500 SE	NBP1-30045MFV500
mFluor Violet 610 SE	NBP1-30045MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-30045SS
Azide and BSA Free	NBP2-80920

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NBP1-18605

Prox1 Antibody

(1 Review)

(5 Publications)

0.2 mg

NBP1-18605

Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale?=?30 um. n?=?3 brains. E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale?=?30 um. n?=?3 brains. E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic ( red arrows) in the meninges but nuclear in neurons ( blue arrows). DAPI marks the nuclei in blue. Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00401-019-02091-z) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-24560

Prox1 Antibody (CL13472)

100 ul

NBP3-24560

Staining of human prostate shows no positivity in glandular cells as expected.

Staining of human skin shows no positivity in squamous epithelial cells as expected.

Staining of human pancreas shows weak to moderate nuclear positivity in exocrine glandular cells.

Staining of mouse hippocampus shows strong nuclear positivity in the granular cell layer of the dentate gyrus.

Western blot analysis in human cell line SH-SY5Y.

Staining of human duodenum shows moderate nuclear positivity in a subset of glandular cells.

Staining of rat hippocampus shows strong nuclear positivity in the granular cell layer of the dentate gyrus.

Reactivity:

Hu, Mu, Rt, Rt

Species Glossary

Reactivity: Hu, Mu, Rt, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-18606

Prox1 Antibody

0.05 mg

NBP1-18606

Western Blot: Prox1 Antibody [NBP1-18606] - Western Blot analysis with Prox-1 Antibody.

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18606] - Double IF stanining of human Prox-1 and CD31 in a mix of HUVEC and HDLEC with Prox1 Antibody (green).

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18606] - Immunofluorescence staining (green) of primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Prox-1 (1ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 was used 1:400

Reactivity:

Hu, Ch, Eq

Species Glossary

Reactivity: Hu, Ch, Eq

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-16739

Prox1 Antibody (9I9A7)

100 ul

NBP3-16739

Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739] - Western blot analysis of extracts of various cell lines, using Prox1 Rabbit mAb (NBP3-16739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739] - Immunohistochemistry of paraffin-embedded human placenta using Prox1 Rabbit mAb (NBP3-16739) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-19747

Prox1 Antibody (S02-8H5)

100 ul

NBP3-19747

Western Blot: Prox1 Antibody (S02-8H5) [NBP3-19747] - Western blot detection of Prox1 in C6 cell lysates using NBP3-19747 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 85kDa.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-55154

Prox1 Antibody

100 ul

NBP2-55154

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55154] - Staining of human cell line Hep G2 shows localization to nucleoplasm.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-55222

Prox1 Antibody

100 ul

NBP2-55222

Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55222] - Staining of human cell line Hep G2 shows localization to nucleoplasm.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-24561

Prox1 Antibody (CL13474)

100 ul

NBP3-24561

Staining in SH-SY5Y cell line with Anti-PROX1 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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