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PRMT6 Antibody (PCRP-PRMT6-2C9)

Images

 
Immunocytochemistry/ Immunofluorescence: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using PRMT6 antibody (PCRP-PRMT6-2C9) followed by goat anti-mouse ...read more
Flow Cytometry: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Flow Cytometric Analysis of PFA-fixed HeLa cells. PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9) followed by goat anti- Mouse IgG-CF488 (blue); unstained ...read more
Protein Array: PRMT6 Antibody (PCRP-PRMT6-2C9) [NBP3-07656] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PRMT6 Mouse Monoclonal Antibody (PCRP-PRMT6-2C9).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IP, MA
Clone
PCRP-PRMT6-2C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PRMT6 Antibody (PCRP-PRMT6-2C9) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08393)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human PRMT6 protein (Uniprot: Q96LA8 )
Localization
Nucleus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRMT6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/100-500 ug protein
  • Protein Array
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for PRMT6 Antibody (PCRP-PRMT6-2C9)

  • EC 2.1.1.-
  • EC 2.1.1.125
  • FLJ10559
  • FLJ51477
  • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6
  • Histone-arginine N-methyltransferase PRMT6
  • HMT1 hnRNP methyltransferase-like 6 (S. cerevisiae)
  • HMT1 hnRNP methyltransferase-like 6
  • HRMT1L6
  • PRMT6
  • protein arginine methyltransferase 6
  • protein arginine N-methyltransferase 6

Background

PRMT6 severely impairs the function of HIV-1 Rev. PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for PRMT6 Antibody (NBP3-07656) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for PRMT6 Antibody (NBP3-07656)

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Blogs on PRMT6.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

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Bioinformatics

Gene Symbol PRMT6
Uniprot