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PRMT3 Antibody

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Western Blot: PRMT3 Antibody [NBP2-19933] - PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation ...read more
Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Western Blot: PRMT3 Antibody [NBP2-19933] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: PRMT3 Antibody [NBP2-19933] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRMT3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PRMT3. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rat (92%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:10000
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PRMT3 Antibody

  • EC 2.1.1
  • EC 2.1.1.-
  • EC 2.1.1.319
  • heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
  • Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
  • HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae)
  • HMT1 hnRNP methyltransferase-like 3
  • HRMT1L3
  • PRMT3
  • protein arginine methyltransferase 3
  • protein arginine N-methyltransferase 3

Background

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-19933

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Bioinformatics

Gene Symbol PRMT3