![Western Blot: PRMT6 Antibody (JE36-57) [NBP3-32854] - Western blot analysis of PRMT6 on different lysates with Rabbit anti-PRMT6 antibody (NBP3-32854) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate (20 ug/Lane) Lane 2: Mouse kidney tissue lysate (20 ug/Lane) Lane 3: Mouse testis tissue lysate (20 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Lane 5: Rat testis tissue lysate (20 ug/Lane) Lane 6: 293T cell lysate (10 ug/Lane) Lane 7: HEK-293 cell lysate (10 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa (Unknown band detected in mouse tissue) Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32854) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32854_rabbit-prmt6-mab-je36-57-288202412442935.jpg "Western Blot: PRMT6 Antibody (JE36-57) [NBP3-32854] - Western blot analysis of PRMT6 on different lysates with Rabbit anti-PRMT6 antibody (NBP3-32854) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate (20 ug/Lane) Lane 2: Mouse kidney tissue lysate (20 ug/Lane) Lane 3: Mouse testis tissue lysate (20 ug/Lane) Lane 4: Rat kidney tissue lysate (20 ug/Lane) Lane 5: Rat testis tissue lysate (20 ug/Lane) Lane 6: 293T cell lysate (10 ug/Lane) Lane 7: HEK-293 cell lysate (10 ug/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa (Unknown band detected in mouse tissue) Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32854) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clone | JE36-57 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide within Human PRMT6 aa 191-240 / 375. (Uniprot: Q96LA8) |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PRMT6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRMT6 Antibody (NBP3-32854)Find related products by research area.
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PRMT6: One Function, Many Roles Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P... Read full blog post. |
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| Gene Symbol | PRMT6 |
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