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PP2C beta/PPM1B Antibody

Images

 
Western Blot: PP2C beta/PPM1B Antibody [NBP1-87250] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA ...read more
Immunocytochemistry/ Immunofluorescence: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human cell line A-431 shows positivity in nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PP2C beta/PPM1B Antibody [NBP1-87250] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PP2C beta/PPM1B Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAEL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPM1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PP2C beta/PPM1B Protein (NBP1-87250PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (85%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PP2C beta/PPM1B Antibody

  • EC 3.1.3.16
  • MGC21657
  • PP2C beta
  • Pp2c2
  • PP2CB
  • PP2C-beta
  • PP2C-beta-X
  • PP2CBmagnesium-dependent, beta isoform
  • PPM1B
  • protein phosphatase 1B
  • Protein phosphatase 2C isoform beta
  • protein phosphatase 2C-like protein
  • protein phosphatase, Mg2+/Mn2+ dependent, 1B

Background

PPM1B is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPM1B