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HLCS Antibody (OTI1E4)

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Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.
Western Blot: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HLCS mouse monoclonal antibody.
Flow Cytometry: HLCS Antibody (1E4) [NBP2-01859] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HLCS antibody, and then analyzed by ...read more
Immunohistochemistry-Paraffin: HLCS Antibody (1E4) [NBP2-01859] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70893AF350
Alexa Fluor 405
NBP2-70893AF405
Alexa Fluor 488
NBP2-70893AF488
Alexa Fluor 532
NBP2-70893AF532
Alexa Fluor 594
NBP2-70893AF594
Alexa Fluor 647
NBP2-70893AF647
Alexa Fluor 700
NBP2-70893AF700
Alexa Fluor 750
NBP2-70893AF750
Allophycocyanin
NBP2-70893APC
Biotin
NBP2-70893B
DyLight 350
NBP2-70893UV
DyLight 405
NBP2-70893V
DyLight 488
NBP2-70893G
DyLight 550
NBP2-70893R
DyLight 594
NBP2-70893DL594
DyLight 650
NBP2-70893C
DyLight 680
NBP2-70893FR
DyLight 755
NBP2-70893IR
FITC
NBP2-70893F
HRP
NBP2-70893H
Janelia Fluor 549
NBP2-70893JF549
Janelia Fluor 646
NBP2-70893JF646
PE
NBP2-70893PE
PerCP
NBP2-70893PCP
mFluor Violet 450 SE
NBP2-70893MFV450
mFluor Violet 500 SE
NBP2-70893MFV500
mFluor Violet 610 SE
NBP2-70893MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70893
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HLCS Antibody (OTI1E4) Summary

Immunogen
Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLCS
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HLCS Antibody (OTI1E4)

  • Biotin apo-protein ligase
  • biotin--protein ligase
  • EC 6.3.4.-
  • HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase)
  • holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Background

This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(OTI1E4)
NBP2-01859
HLCS Antibody (OTI1E4)
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Bioinformatics

Gene Symbol HLCS
Entrez